Recombinant Mouse IFN-epsilon Protein

R&D Systems | Catalog # 9147-ME

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse IFN-epsilon Protein (9147-ME)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q80ZF2

Applications

Bioactivity
View all IFN-epsilon Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse IFN-epsilon protein
Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag

Purity

>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

21 kDa

SDS-PAGE

20 kDa, reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 6.00-60.0 ng/mL.

Scientific Data Images for Recombinant Mouse IFN-epsilon Protein

Recombinant Mouse IFN-epsilon Protein Bioactivity

Recombinant Mouse IFN-epsilon Protein Bioactivity

Recombinant Mouse IFN-epsilon (Catalog # 9147-ME) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 6.00-60.0 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9147-ME
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9147-ME/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS
Reconstitution Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IFN-epsilon

Interferon-epsilon (IFN-epsilon ) is a secreted, approximately 18 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-epsilon shares 55% amino acid sequence identity with human and rat IFN-epsilon, respectively. IFN-epsilon signals through IFN-alpha / beta R1 and IFN-alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen
(2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8+ T cells to sites of virus infection (5, 7).

References

  1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
  5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
  6. Hermant, P. et al. (2013) PLoS One 8:e71320.
  7. Day, S.L. et al. (2008) J. Immunol. 180:7158.

Long Name

Interferon epsilon

Alternate Names

Ifn-tau-11, IFNE, IFNepsilon, IFNT1, INFE1

Entrez Gene IDs

338376 (Human); 230405 (Mouse); 298225 (Rat)

Gene Symbol

IFNE

UniProt

Q80ZF2

Additional IFN-epsilon Products

Product Documents for Recombinant Mouse IFN-epsilon Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 9147-ME (with carrier)
Download SDS 9147-ME/CF (carrier free)

Product Specific Notices for Recombinant Mouse IFN-epsilon Protein

For research use only

Related Research Areas

Interferons

Customer Reviews for Recombinant Mouse IFN-epsilon Protein (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Mouse IFN-epsilon Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Recombinant Mouse IFN-epsilon Protein
    Name: Anonymous
    Verified Customer | Posted 10/23/2020

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes