Recombinant Mouse IFN-epsilon Protein

  
  • Purity
    >75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.2-1 ng/mL.
  • Source
    E. coli-derived Leu22-Pro192, with N-terminal Met and C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    20 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9147-ME
 
9147-ME/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS
Reconstitution Reconstitute at 100 μg/mL in water.
Reconstitution Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IFN-epsilon (Catalog # 9147-ME) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1 ng/mL.
Background: IFN-epsilon
Interferon-epsilon (IFN-epsilon ) is a secreted, approximately 18 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-epsilon shares 55% amino acid sequence identity with human and rat IFN-epsilon, respectively. IFN-epsilon signals through IFN-alpha / beta R1 and IFN-alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen
(2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation and the attraction of CD8+ T cells to sites of virus infection (5, 7).
  • References:
    1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
    2. Fung, K.Y. et al. (2013) Science 339:1088.
    3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
    4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
    5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
    6. Hermant, P. et al. (2013) PLoS One 8:e71320.
    7. Day, S.L. et al. (2008) J. Immunol. 180:7158.
  • Long Name:
    Interferon epsilon
  • Entrez Gene IDs:
    338376 (Human); 230405 (Mouse); 298225 (Rat)
  • Alternate Names:
    IFNE; IFN-E; IFNE1PRO655; IFNepsilon; IFN-epsilon; IFNT1; Ifn-tau-11; INFE1; interferon epsilon 1; interferon epsilon; Interferon epsilon-1; interferon tau-1; interferon, epsilon; interferon-epsilon; MGC119018; MGC119020
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 25  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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