Recombinant Mouse IFNAB Protein, CF

Catalog # Availability Size / Price Qty
10151-IF-010
10151-IF-050
Recombinant Mouse IFNAB Protein, CF Bioactivity
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Recombinant Mouse IFNAB Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.6-6 pg/mL
Source
Human embryonic kidney cell, HEK293-derived mouse IFNAB protein
Cys24-Glu190
Accession #
N-terminal Sequence
Analysis
Cys24
Predicted Molecular Mass
19 kDa
SDS-PAGE
17-25 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10151-IF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse IFNAB (Catalog # 10151-IF) suppressesviral activity on L-929 mouse fibroblast cells infected withencephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 pg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse IFNAB(Catalog # 10151-IF) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 17-25 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFNAB

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN alpha subtypes and the various IFN alpha share about 80% sequence homology among them (4, 5). The mouse IFNAB consists of 190 amino acids (aa) including a 23 aa signal peptide and a 167 aa IFNAB main chain. It was found that IFNAB plays an important role in the regulation of DNA synthesis in colong-stimulating factor (CSF)-1-stimulated macrophage, as well as mediates the inhibitory efffects of LPS and TNF alpha (6). IFN regulatory factor 2 (IRF-2) controls IFNAB signaling and the absence of its negative regulation selectively affects development of the CD8 alpha - myeloid DC population (7).


References
  1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. Hamilton JA, et al. (1996) J Immunol. 156(7):2553.
  7. Kenya H, et al. (2004) Proc Natl Acad Sci U S A. 101(8):2416.
Long Name
Interferon alpha B
Entrez Gene IDs
15974 (Mouse)
Alternate Names
IFNAB

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