Recombinant Mouse IL-19 Protein, CF 2915-IL-025/CF: R&D Systems

Recombinant Mouse IL-19 Protein, CF

(6 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.2-0.8 ng/mL.
  • Source
    E. coli-derived Leu25-Ala176, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    17.7 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2915-IL/CF
 
2915-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24)(2 - 4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). Based on human studies, it is expected to be secreted as a glycosylated monomer, 35 - 45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity to unpublished Genbank sequences for rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL-19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

  • References:
    1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.
    2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
    3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
    4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615. 
    5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
    6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
    7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
    8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
    9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
    10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
    11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
    13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
    14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
    15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.
  • Long Name:
    Interleukin 19
  • Entrez Gene IDs:
    29949 (Human); 329244 (Mouse)
  • Alternate Names:
    IL-10C; IL19; IL-19; interleukin 19; MDA1; melanoma differentiation associated protein-like protein; NG.1Melanoma differentiation-associated protein-like protein; ZMDA1interleukin-19
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Molecular Mechanisms of Airway Hyperresponsiveness in a Murine Model of Steroid-Resistant Airway Inflammation.
    Authors: Manni M, Mandalapu S, McHugh K, Elloso M, Dudas P, Alcorn J
    J Immunol, 2016;196(3):963-77.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. Interleukin-19 acts as a negative autocrine regulator of activated microglia.
    Authors: Horiuchi H, Parajuli B, Wang Y, Azuma Y, Mizuno T, Takeuchi H, Suzumura A
    PLoS ONE, 2015;10(3):e0118640.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012;7(7):e40343.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Interleukin-19: a constituent of the regulome that controls antigen presenting cells in the lungs and airway responses to microbial products.
    Authors: Hoffman C, Park SH, Daley E, Emson C, Louten J, Sisco M, de Waal Malefyt R, Grunig G
    PLoS ONE, 2011;6(11):e27629.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  6. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo

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