Recombinant Mouse IL-20 Protein

  (5 citations)     
Datasheet / CoA / SDS
Product Details
Citations (5)
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  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.5‑2.5 ng/mL.
  • Source
    E. coli-derived mouse IL-20 protein
    Leu25-Leu176, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    17.7 kDa
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1204-ML
 
1204-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Background: IL-20

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL‑20 R beta. The second complex is composed of IL-22 R and IL‑20 R beta. Whereas the IL-22 R/IL-20 R beta  complex is shared with IL-24/mda-7, the IL‑20 R alpha /IL‑20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1 - 6).

  • References:
    1. Blumberg, H. et al. (2001) Cell 104:9.
    2. Liu, L. et al. (2003) Blood 102:3206.
    3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
    4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
    5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
    6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
  • Long Name:
    Interleukin 20
  • Entrez Gene IDs:
    50604 (Human); 58181 (Mouse)
  • Alternate Names:
    Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. IL-20 promotes epithelial healing of the injured mouse cornea
    Exp. Eye Res., 2016;154(0):22-29.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. IL-20 promotes epithelial healing of the injured mouse cornea
    Authors: Alan R Burns
    Exp. Eye Res., 2016;154(0):22-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  3. Interleukin-22 alleviates metabolic disorders and restores mucosal immunity in diabetes.
    Authors: Wang, Xiaoting, Ota, Naruhisa, Manzanillo, Paolo, Kates, Lance, Zavala-Solorio, Jose, Eidenschenk, Celine, Zhang, Juan, Lesch, Justin, Lee, Wyne P, Ross, Jed, Diehl, Lauri, van Bruggen, Nicholas, Kolumam, Ganesh, Ouyang, Wenjun
    Nature, 2014;514(7521):237-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo

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