Recombinant Mouse IL-27 (NS0-expressed) Protein

(26 citations)
(1 Review)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 0.6-6 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse EBI3
    (Tyr19-Pro228)
    Accession # O35228
    GGGSGGGSGGGSGGGS Mouse p28
    (Phe29-Ser234)
    Accession # Q8K3I6
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr19
  • Predicted Molecular Mass
    48.7 kDa
  • SDS-PAGE
    60-65 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2799-ML
 
2799-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

Recombinant Mouse IL-27 (Catalog # 2799‑ML) demonstrates anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 ng/mL.

1 μg/lane of Recombinant Mouse IL-27 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 61 kDa.
Background: IL-27

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL-12), IL-27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N-linked glycosylation site and four alpha -helices, placing it in the IL-6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (7, 9, 10). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction (11).

  • References:
    1. Boulay, J-L. et al. (2003) Immunity 19:159.
    2. Trinchieri, G. et al. (2003) Immunity 19:641.
    3. Murakami, M. et al. (2004) Growth Factors 22:75.
    4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Expert Opin. Biol. Ther. 3:715.
    5. Nomura, H. et al. (1997) GenBank Accession # O35228.
    6. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl). 193:1.
    7. Pflanz, S. et al. (2002) Immunity 16:779.
    8. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
    9. Pflanz, S. et al. (2004) J. Immunol. 172: 2225.
    10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
    11. Hawlish, H. et al. (2005) Immunity 22:415.
  • Long Name:
    Interleukin 27
  • Entrez Gene IDs:
    246778 (Human); 246779 (Mouse)
  • Alternate Names:
    IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Treg-specific IL-27R? deletion uncovers a key role for IL-27 in Treg function to control autoimmunity
    Authors: J Do, D Kim, S Kim, A Valentin-T, N Dvorina, E Jang, V Nagarajave, TM DeSilva, X Li, AH Ting, DAA Vignali, SA Stohlman, WM Baldwin, B Min
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. The STAT4/MLL1 Epigenetic Axis Regulates the Antimicrobial Functions of Murine Macrophages
    Authors: WF Carson, KA Cavassani, EM Soares, S Hirai, NA Kittan, MA Schaller, MM Scola, A Joshi, A Matsukawa, DM Aronoff, CN Johnson, Y Dou, KA Gallagher, SL Kunkel
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Bioassay
  4. Balanced Bcl-3 expression in murine CD4(+) T cells is required for generation of encephalitogenic Th17 cells
    Authors: IA Mufazalov, J Kuschmann, D Andruszews, J Masri, LA Gabriel, P Adams, S Reissig, N Hövelmeyer, A Waisman
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes
    Sci Rep, 2016;6(0):30448.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  6. IL-27 Directly Enhances Germinal Center B Cell Activity and Potentiates Lupus in Sanroque Mice
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Differential Production of Type I IFN Determines the Reciprocal Levels of IL-10 and Proinflammatory Cytokines Produced by C57BL/6 and BALB/c Macrophages
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  8. Interleukin-27 is elevated in sepsis-induced myocardial dysfunction and mediates inflammation
    Cytokine, 2016;88(0):1-11.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  9. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
    Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
    Nat Commun, 2015;6(0):6329.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: Bioassay
  10. An IL-27/NFIL3 signalling axis drives Tim-3 and IL-10 expression and T-cell dysfunction.
    Authors: Zhu C, Sakuishi K, Xiao S, Sun Z, Zaghouani S, Gu G, Wang C, Tan D, Wu C, Rangachari M, Pertel T, Jin H, Ahmed R, Anderson A, Kuchroo V
    Nat Commun, 2015;6(0):6072.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Role of Blimp-1 in programing Th effector cells into IL-10 producers.
    Authors: Neumann C, Heinrich F, Neumann K, Junghans V, Mashreghi M, Ahlers J, Janke M, Rudolph C, Mockel-Tenbrinck N, Kuhl A, Heimesaat M, Esser C, Im S, Radbruch A, Rutz S, Scheffold A
    J Exp Med, 2014;211(9):1807-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Timed action of IL-27 protects from immunopathology while preserving defense in influenza.
    Authors: Liu F, Kenngott E, Schroter M, Kuhl A, Jennrich S, Watzlawick R, Hoffmann U, Wolff T, Norley S, Scheffold A, Stumhofer J, Saris C, Schwab J, Hunter C, Debes G, Hamann A
    PLoS Pathog, 2014;10(5):e1004110.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  13. IL-27 limits central nervous system viral clearance by promoting IL-10 and enhances demyelination.
    Authors: de Aquino M, Kapil P, Hinton D, Phares T, Puntambekar S, Savarin C, Bergmann C, Stohlman S
    J Immunol, 2014;193(1):285-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting RORgammat and STAT3.
    Authors: Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, Zhao Q, Zhao L, Wu Z, Yin Z
    J Immunol, 2014;192(12):5599-609.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. IL-21 promotes CD8+ CTL activity via the transcription factor T-bet.
    Authors: Sutherland A, Joller N, Michaud M, Liu S, Kuchroo V, Grusby M
    J Immunol, 2013;190(8):3977-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. IL-6 activates STAT5 in T cells.
    Authors: Tormo AJ, Letellier MC, Sharma M, Elson G, Crabe S, Gauchat JF
    Cytokine, 2012;60(0):575.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Interleukin-27 and interferon-gamma are involved in regulation of autoimmune arthritis.
    Authors: Rajaiah R, Puttabyatappa M, Polumuri SK, Moudgil KD
    J. Biol. Chem., 2011;286(4):2817-25.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  19. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  20. Essential role for IL-27 receptor signaling in prevention of Th1-mediated immunopathology during malaria infection.
    Authors: Findlay EG, Greig R, Stumhofer JS
    J. Immunol., 2010;185(4):2482-92.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  21. Cutting edge: IL-27 induces the transcription factor c-Maf, cytokine IL-21, and the costimulatory receptor ICOS that coordinately act together to promote differentiation of IL-10-producing Tr1 cells.
    Authors: Pot C, Jin H, Awasthi A
    J. Immunol., 2009;183(2):797-801.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance.
    Authors: Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S
    Cancer Res., 2009;69(13):5505-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. The type I IFN induction pathway constrains Th17-mediated autoimmune inflammation in mice.
    Authors: Guo B, Chang EY, Cheng G
    J. Clin. Invest., 2008;118(5):1680-90.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  24. Amelioration of delayed-type hypersensitivity responses by IL-27 administration.
    Authors: Miyazaki Y, Shimanoe Y, Wang S, Yoshida H
    Biochem. Biophys. Res. Commun., 2008;373(3):397-402.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  25. Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
    Authors: Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, Yu S, Saris CJ, Gran B, Ciric B, Rostami A
    Nat. Immunol., 2007;8(12):1372-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis.
    Authors: Fitzgerald DC, Ciric B, Touil T, Harle H, Grammatikopolou J, Das Sarma J, Gran B, Zhang GX, Rostami A
    J. Immunol., 2007;179(5):3268-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 26 Citations
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Average Rating: 5 (Based on 1 review)

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T cell differentiation Recombinant Mouse IL-27 (NS0-expressed) Protein 2799-ML
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T cell differentiation Recombinant Mouse IL-27 (NS0-expressed) Protein 2799-ML
T cell differentiation: Recombinant Mouse IL-27 (NS0-expressed) Protein [2799-ML]

Summary

ApplicationT cell differentiation
Reason for RatingWorks well in inducing IFNg and IL-10 expression in naive mouse T cells.

Other Experimental Details

Other Experimental DetailsNaive (CD4+CD44+CD62L++CD25-) mouse T cells were stimulated with anti-CD3, anti-CD28, IL-2 and 50 ng/mL IL-27. IFNg and IL-10 secretion was analyzed in the culture supernatants 5 days later by multiplex assays.

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