Recombinant Mouse IL-3 Protein

(82 citations)
(3 Reviews)
  
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Holmes, K.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The ED50 for this effect is 20-100 pg/mL.
  • Source
    E. coli-derived Asp33-Cys166, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp33
  • Predicted Molecular Mass
    15 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
403-ML
 
403-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 20-100 pg/mL.
1 μg/lane of Recombinant Mouse IL-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 15 kDa.
Background: IL-3

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.

IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells.

  • References:
    1. Yokota, T. et al., 1984, Proc. Natl. Acad. Sci. USA 81:1070.
    2. Fung, M.C. et al., 1984, Nature 307:233.
    3. Miyatake, S. et al., 1985, Proc. Natl. Acad. Sci. USA 82:316.
  • Long Name:
    Interleukin 3
  • Entrez Gene IDs:
    3562 (Human); 16187 (Mouse); 24495 (Rat)
  • Alternate Names:
    Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

82 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Proteomic and Metabolomic Characterization of a Mammalian Cellular Transition from Quiescence to Proliferation
    Authors: HJ Lee, MP Jedrychows, A Vinayagam, N Wu, N Shyh-Chang, Y Hu, C Min-Wen, JK Moore, JM Asara, CA Lyssiotis, N Perrimon, SP Gygi, LC Cantley, MW Kirschner
    Cell Rep, 2017;20(3):721-736.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Funiculosin variants and phosphorylated derivatives promote innate immune responses via the Toll-like receptor 4/myeloid differentiation factor-2 complex
    Authors: N Okamoto, K Mizote, H Honda, A Saeki, Y Watanabe, T Yamaguchi-, R Fukui, N Tanimura, Y Motoi, S Akashi-Tak, T Kato, S Fujishita, T Kimura, U Ohto, T Shimizu, T Hirokawa, K Miyake, K Fukase, Y Fujimoto, Y Nagai, K Takatsu
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Radiation Induced Apoptosis of Murine Bone Marrow Cells Is Independent of Early Growth Response 1 (EGR1)
    Authors: KZ Oben, BW Gachuki, SS Alhakeem, MK McKenna, Y Liang, DK St Clair, VM Rangnekar, S Bondada
    PLoS ONE, 2017;12(1):e0169767.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Generation and characterization of an antagonistic monoclonal antibody against an extracellular domain of mouse DP2 (CRTH2/GPR44) receptors for prostaglandin D2
    Authors: N Nagata, H Iwanari, H Kumagai, O Kusano-Ara, Y Ikeda, K Aritake, T Hamakubo, Y Urade
    PLoS ONE, 2017;12(4):e0175452.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Acquired resistance to LY2874455 in FGFR2-amplified gastric cancer through an emergence of novel FGFR2-ACSL5 fusion
    Authors: SY Kim, T Ahn, H Bang, JS Ham, J Kim, ST Kim, J Jang, M Shim, SY Kang, SH Park, BH Min, H Lee, WK Kang, KM Kim, W Park, J Lee
    Oncotarget, 2017;8(9):15014-15022.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. A Bach2-Cebp Gene Regulatory Network for the Commitment of Multipotent Hematopoietic Progenitors
    Authors: A Itoh-Nakad, M Matsumoto, H Kato, J Sasaki, Y Uehara, Y Sato, R Ebina-Shib, M Morooka, R Funayama, K Nakayama, K Ochiai, A Muto, K Igarashi
    Cell Rep, 2017;18(10):2401-2414.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Mesenchymal Cell Reprogramming in Experimental MPLW515L Mouse Model of Myelofibrosis
    Authors: Y Han, L Yue, M Wei, X Ren, Z Shao, L Zhang, RL Levine, PK Epling-Bur
    PLoS ONE, 2017;12(1):e0166014.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Engineering and Functional Characterization of Fusion Genes Identifies Novel Oncogenic Drivers of Cancer
    Authors: H Lu, N Villafane, T Dogruluk, CL Grzeskowia, K Kong, YH Tsang, O Zagorodna, A Pantazi, L Yang, NJ Neill, YW Kim, CJ Creighton, RG Verhaak, GB Mills, P Park, RS Kucherlapa, KL Scott
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. Interaction of primary mast cells with Borrelia burgdorferi (sensu stricto): role in transmission and dissemination in C57BL/6 mice
    Authors: Q Bernard, Z Wang, A Di Nardo, N Boulanger
    Parasit Vectors, 2017;10(1):313.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. A novel, somatic, transforming mutation in the extracellular domain of Epidermal Growth Factor Receptor identified in myeloproliferative neoplasm
    Authors: DA Casolari, T Nguyen, CM Butcher, DG Iarossi, CN Hahn, SC Bray, P Neufing, WT Parker, J Feng, KZY Maung, A Wee, L Vidovic, CH Kok, PG Bardy, S Branford, ID Lewis, SW Lane, HS Scott, DM Ross, RJ D'Andrea
    Sci Rep, 2017;7(1):2467.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Lipopolysaccharide/TLR4 Stimulates IL-13 Production through a MyD88-BLT2-Linked Cascade in Mast Cells, Potentially Contributing to the Allergic Response
    Authors: AJ Lee, M Ro, KJ Cho, JH Kim
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Massively parallel nanowell-based single-cell gene expression profiling
    Authors: LD Goldstein, YJ Chen, J Dunne, A Mir, H Hubschle, J Guillory, W Yuan, J Zhang, J Stinson, B Jaiswal, KB Pahuja, I Mann, T Schaal, L Chan, S Anandakris, CW Lin, P Espinoza, S Husain, H Shapiro, K Swaminatha, S Wei, M Srinivasan, S Seshagiri, Z Modrusan
    BMC Genomics, 2017;18(1):519.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Quantitative trait gene Slit2 positively regulates murine hematopoietic stem cell numbers
    Sci Rep, 2016;6(0):31412.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors
    Nat Commun, 2016;7(0):13396.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Basophils promote tumor rejection via chemotaxis and infiltration of CD8+ T cells
    Cancer Res., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Pot1 OB-fold mutations unleash telomere instability to initiate tumorigenesis
    Oncogene, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Cycloheximide Can Induce Bax/Bak Dependent Myeloid Cell Death Independently of Multiple BH3-Only Proteins
    PLoS ONE, 2016;11(11):e0164003.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Chronic myelogenous leukemia initiating cells require Polycomb group protein EZH2
    Cancer Discov, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Mast cell desensitization inhibits calcium flux and aberrantly remodels actin
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Gene Therapy Induces Antigen-Specific Tolerance in Experimental Collagen-Induced Arthritis
    PLoS ONE, 2016;11(5):e0154630.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. iNKT Cells Are Responsible for the Apoptotic Reduction of Basophils That Mediate Th2 Immune Responses Elicited by Papain in Mice Following ?PGA Stimulation
    Authors: HJ Park, SW Lee, SH Park, S Hong
    PLoS ONE, 2016;11(4):e0152189.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. A functional bioassay to determine the activity of anti-VEGF antibody therapy in blood of patients with cancer
    Br J Cancer, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Grb10 is involved in BCR-ABL-positive leukemia in mice.
    Authors: Illert, A L, Albers, C, Kreutmair, S, Leischner, H, Peschel, C, Miething, C, Duyster, J
    Leukemia, 2015;29(4):858-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Ubiquitous Over-Expression of Chromatin Remodeling Factor SRG3 Ameliorates the T Cell-Mediated Exacerbation of EAE by Modulating the Phenotypes of both Dendritic Cells and Macrophages.
    Authors: Lee S, Park H, Jeon S, Lee C, Seong R, Park S, Hong S
    PLoS ONE, 2015;10(7):e0132329.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy.
    Authors: Schumacher A, Denecke B, Braunschweig T, Stahlschmidt J, Ziegler S, Brandenburg L, Stope M, Martincuks A, Vogt M, Gortz D, Camporeale A, Poli V, Muller-Newen G, Brummendorf T, Ziegler P
    J Hematol Oncol, 2015;8(0):64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence.
    Authors: Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, Ross S, Sakamoto K, Zon L, Schlaeger T, Orkin S, Nakano A, Daley G
    J Exp Med, 2015;212(5):633-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Coordinate regulation of residual bone marrow function by paracrine trafficking of AML exosomes.
    Authors: Huan J, Hornick N, Goloviznina N, Kamimae-Lanning A, David L, Wilmarth P, Mori T, Chevillet J, Narla A, Roberts C, Loriaux M, Chang B, Kurre P
    Leukemia, 2015;29(12):2285-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor.
    Authors: Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K
    Stem Cells, 2015;33(3):976-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015;15(0):105.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  31. CD252 regulates mast cell mediated, CD1d-restricted NKT-cell activation in mice.
    Authors: Gonzalez Roldan N, Orinska Z, Ewers H, Bulfone-Paus S
    Eur J Immunol, 2015;46(2):432-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. The role of Lin28b in myeloid and mast cell differentiation and mast cell malignancy.
    Authors: Wang L, Rao T, Rowe R, Nguyen P, Sullivan J, Pearson D, Doulatov S, Wu L, Lindsley R, Zhu H, DeAngelo D, Daley G, Wagers A
    Leukemia, 2015;29(6):1320-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  33. HoxA10 Terminates Emergency Granulopoiesis by Increasing Expression of Triad1.
    Authors: Wang H, Bei L, Shah C, Hu L, Eklund E
    J Immunol, 2015;194(11):5375-87.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. Hematopoietic stem/progenitor cells express several functional sex hormone receptors-novel evidence for a potential developmental link between hematopoiesis and primordial germ cells.
    Authors: Mierzejewska K, Borkowska S, Suszynska E, Suszynska M, Poniewierska-Baran A, Maj M, Pedziwiatr D, Adamiak M, Abdel-Latif A, Kakar S, Ratajczak J, Kucia M, Ratajczak M
    Stem Cells Dev, 2015;24(8):927-37.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  35. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells.
    Authors: Luyten A, Zang C, Liu X, Shivdasani R
    Genes Dev, 2014;28(16):1827-39.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  36. The adult livers of immunodeficient mice support human hematopoiesis: evidence for a hepatic mast cell population that develops early in human ontogeny.
    Authors: Muench M, Beyer A, Fomin M, Thakker R, Mulvaney U, Nakamura M, Suemizu H, Barcena A
    PLoS ONE, 2014;9(5):e97312.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  37. Embryonic stem cell-derived haematopoietic progenitor cells down-regulate the CD3 xi chain on T cells, abrogating alloreactive T cells.
    Authors: Kim E, Miyake B, Aggarwal M, Voetlause R, Griffith M, Zavazava N
    Immunology, 2014;142(3):421-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. TCR affinity and tolerance mechanisms converge to shape T cell diabetogenic potential.
    Authors: Bettini M, Blanchfield L, Castellaw A, Zhang Q, Nakayama M, Smeltzer M, Zhang H, Hogquist K, Evavold B, Vignali D
    J Immunol, 2014;193(2):571-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  39. A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
    Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
    Sci Rep, 2014;4(0):6222.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  40. The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface.
    Authors: Lee F, Luevano M, Veys P, Yong K, Madrigal A, Shaw B, Saudemont A
    PLoS ONE, 2014;9(7):e103254.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. CD41 is a reliable identification and activation marker for murine basophils in the steady state and during helminth and malarial infections.
    Authors: Bakocevic N, Claser C, Yoshikawa S, Jones L, Chew S, Goh C, Malleret B, Larbi A, Ginhoux F, de Lafaille M, Karasuyama H, Renia L, Ng L
    Eur J Immunol, 2014;44(6):1823-34.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo injection
  42. Expression of podocalyxin separates the hematopoietic and vascular potentials of mouse embryonic stem cell-derived mesoderm.
    Authors: Zhang H, Nieves J, Fraser S, Isern J, Douvaras P, Papatsenko D, D'Souza S, Lemischka I, Dyer M, Baron M
    Stem Cells, 2014;32(1):191-203.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  43. Antineoplastic activity of rinvanil and phenylacetylrinvanil in leukaemia cell lines.
    Authors: Luviano A, Aguiniga-Sanchez I, Demare P, Tiburcio R, Ledesma-Martinez E, Santiago-Osorio E, Regla I
    Oncol Lett, 2014;7(5):1651-1656.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  44. Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells.
    Authors: Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata K, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T
    Blood, 2014;123(25):3932-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  45. Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells.
    Authors: Mabe, Satoru, Nagamune, Teruyuki, Kawahara, Masahiro
    Sci Rep, 2014;4(0):6127.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  46. Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression.
    Authors: Wallington-Beddoe C, Powell J, Tong D, Pitson S, Bradstock K, Bendall L
    Cancer Res, 2014;74(10):2803-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  47. Mast cells contribute to bleomycin-induced lung inflammation and injury in mice through a chymase/mast cell protease 4-dependent mechanism.
    Authors: Reber L, Daubeuf F, Pejler G, Abrink M, Frossard N
    J Immunol, 2014;192(4):1847-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  48. Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells.
    Authors: Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit M, Blanchet X, von Hundelshausen P, Weber C, Forssmann W, Henschler R
    Stem Cells Dev, 2014;23(16):1959-74.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  49. The DNA binding property of PML/RARA but not the integrity of PML nuclear bodies is indispensable for leukemic transformation.
    Authors: Liu X, Yuan H, Peres L, Chen S, Chen Z, de The H, Zhou J, Zhu J
    PLoS ONE, 2014;9(8):e104906.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  50. Targeting oncoprotein stability overcomes drug resistance caused by FLT3 kinase domain mutations.
    Authors: Yu, Chuanjia, Kancha, Rama Kri, Duyster, Justus
    PLoS ONE, 2014;9(5):e97116.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  51. Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow.
    Authors: Hiragun T, Yanase Y, Okabe T, Hiragun M, Kawai M, Hide M
    FEBS Open Bio, 2014;4(0):342-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  52. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
    Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
    Sci Rep, 2014;4(0):5199.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  53. Endothelial cells translate pathogen signals into G-CSF-driven emergency granulopoiesis.
    Authors: Boettcher S, Gerosa R, Radpour R, Bauer J, Ampenberger F, Heikenwalder M, Kopf M, Manz M
    Blood, 2014;124(9):1393-403.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  54. Prostaglandin E2-EP3 signaling induces inflammatory swelling by mast cell activation.
    Authors: Morimoto, Kazushi, Shirata, Naritosh, Taketomi, Yoshitak, Tsuchiya, Soken, Segi-Nishida, Eri, Inazumi, Tomoaki, Kabashima, Kenji, Tanaka, Satoshi, Murakami, Makoto, Narumiya, Shuh, Sugimoto, Yukihiko
    J Immunol, 2014;192(3):1130-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  55. Adipose tissue macrophages promote myelopoiesis and monocytosis in obesity.
    Authors: Nagareddy P, Kraakman M, Masters S, Stirzaker R, Gorman D, Grant R, Dragoljevic D, Hong E, Abdel-Latif A, Smyth S, Choi S, Korner J, Bornfeldt K, Fisher E, Dixit V, Tall A, Goldberg I, Murphy A
    Cell Metab, 2014;19(5):821-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  56. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
    Authors: Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L
    J Exp Med, 2013;210(2):339-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  57. Establishment of a cell-type-specific genetic network by the mediator complex component Med1.
    Authors: Pope, Nathanie, Bresnick, Emery H
    Mol Cell Biol, 2013;33(10):1938-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  58. Thymic stromal lymphopoietin-mediated extramedullary hematopoiesis promotes allergic inflammation.
    Authors: Siracusa M, Saenz S, Wojno E, Kim B, Osborne L, Ziegler C, Benitez A, Ruymann K, Farber D, Sleiman P, Hakonarson H, Cianferoni A, Wang M, Spergel J, Comeau M, Artis D
    Immunity, 2013;39(6):1158-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  59. IL-33 causes selective mast cell tolerance to bacterial cell wall products by inducing IRAK1 degradation.
    Authors: Sandig H, Jobbings C, Roldan N, Whittingham-Dowd J, Orinska Z, Takeuchi O, Akira S, Bulfone-Paus S
    Eur J Immunol, 2013;43(4):979-88.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  60. The leukemia-associated Mll-Ell oncoprotein induces fibroblast growth factor 2 (Fgf2)-dependent cytokine hypersensitivity in myeloid progenitor cells.
    Authors: Shah, Chirag A, Bei, Ling, Wang, Hao, Platanias, Leonidas, Eklund, Elizabet
    J Biol Chem, 2013;288(45):32490-505.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  61. Fas-associated phosphatase 1 (Fap1) influences betacatenin activity in myeloid progenitor cells expressing the Bcr-abl oncogene.
    Authors: Huang W, Bei L, Eklund E
    J Biol Chem, 2013;288(18):12766-76.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  62. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  63. The critical role of mast cell-derived hypoxia-inducible factor-1alpha in human and mice melanoma growth.
    Authors: Jeong H, Oh H, Nam S, Han N, Kim Y, Kim J, Lee S, Kim M, Moon P, Kim H, Oh H
    Int J Cancer, 2013;132(11):2492-501.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  64. Induction of a hemogenic program in mouse fibroblasts.
    Authors: Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K
    Cell Stem Cell, 2013;13(2):205-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  65. Gata2 cis-element is required for hematopoietic stem cell generation in the mammalian embryo.
    Authors: Gao, Xin, Johnson, Kirby D, Chang, Yuan-I, Boyer, Meghan E, Dewey, Colin N, Zhang, Jing, Bresnick, Emery H
    J Exp Med, 2013;210(13):2833-42.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  66. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  67. Cyclin D1 regulates p27(Kip1) stability in B cells.
    Authors: Bustany S, Tchakarska G, Sola B
    Cell. Signal., 2011;23(1):171-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  68. Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells.
    Authors: Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T
    J. Biol. Chem., 2011;286(29):25739-47.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  69. Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells.
    Authors: Kinzfogl J, Hangoc G, Broxmeyer HE
    Blood, 2011;118(3):565-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  70. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  71. Insertional gene activation by lentiviral and gammaretroviral vectors.
    Authors: Bokhoven M, Stephen SL, Knight S, Gevers EF, Robinson IC, Takeuchi Y, Collins MK
    J. Virol., 2009;83(1):283-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  72. MHC class II-dependent basophil-CD4+ T cell interactions promote T(H)2 cytokine-dependent immunity.
    Authors: Perrigoue JG, Saenz SA, Siracusa MC, Allenspach EJ, Taylor BC, Giacomin PR, Nair MG, Du Y, Zaph C, Van Rooijen N, Comeau MR, Pearce EJ, Laufer TM, Artis D
    Nat. Immunol., 2009;10(7):697-705.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  73. Commonly dysregulated genes in murine APL cells.
    Authors: Yuan W, Payton JE, Holt MS, Link DC, Watson MA, DiPersio JF, Ley TJ
    Blood, 2007;109(3):961-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  74. IL-17B and IL-17C are associated with TNF-alpha production and contribute to the exacerbation of inflammatory arthritis.
    Authors: Yamaguchi Y, Fujio K, Shoda H, Okamoto A, Tsuno NH, Takahashi K, Yamamoto K
    J. Immunol., 2007;179(10):7128-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  75. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  76. Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors.
    Authors: Bao YC, Nomura Y, Moon Y, Tonozuka Y, Minoshima Y, Hatori T, Tsuchiya A, Kiyono M, Nosaka T, Nakajima H
    J. Cell Biol., 2006;175(6):937-46.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  77. SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity.
    Authors: Yilmaz OH, Kiel MJ, Morrison SJ
    Blood, 2005;107(3):924-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  78. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005;174(1):215-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  79. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
    Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
    Exp. Hematol., 2004;32(5):441-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  80. Inhibition of FLT3 in MLL. Validation of a therapeutic target identified by gene expression based classification.
    Authors: Armstrong SA, Kung AL, Mabon ME, Silverman LB, Stam RW, Den Boer ML, Pieters R, Kersey JH, Sallan SE, Fletcher JA, Golub TR, Griffin JD, Korsmeyer SJ
    Cancer Cell, 2003;3(2):173-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  81. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
    Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
    Arch. Dermatol. Res., 2001;293(10):508-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  82. Interleukin-6 and the granulocyte colony-stimulating factor receptor are major independent regulators of granulopoiesis in vivo but are not required for lineage commitment or terminal differentiation.
    Authors: Liu F, Poursine-Laurent J, Wu HY, Link DC
    Blood, 1997;90(7):2583-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 82 Citations
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