Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
7728-A5-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Bovine Fibronectin (Catalog # 1030-FN) is coated at 2 μg/mL, Recombinant Mouse Integrin alpha 5 beta 1 binds with an apparent KD <0.5 nM. 
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 5 beta 1 protein
Mouse Integrin alpha 5
Phe45-Asn999 (Gly377Ala)
Accession # P11688
GS Linker Acidic Tail HHHHHH
Mouse Integrin beta 1
(Gln21-Asp728)
Accession # P09055
HP GS Linker Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe45 (Integrin alpha 5) & Gln21 predicted, No results obtained: sequencing might be blocked (Integrin beta 1)
Structure / Form
Noncovalently-linked heterodimer 
Predicted Molecular Mass
113 kDa (Integrin alpha 5) & 86.5 kDa (Integrin beta 1)
SDS-PAGE
115-165 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7728-A5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 5 beta 1

Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin  alpha 5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha 5 shares 91% and 97% aa sequence identity with human and rat alpha 5, respectively. Mature mouse Integrin beta 1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta 1 shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 5/ beta 1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14‑16).

References
  1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
  2. Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.
  3. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
  16. Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.
Entrez Gene IDs
3678 (Human)
Alternate Names
CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha polypeptide; fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 beta 1; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5/SLC9B1 fusion; very late activation protein 5, alpha subunit; VLA-5; VLA5A

Citations for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics
    Authors: MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau
    Sci Rep, 2018;8(1):11497.
    Species: N/A
    Sample Types: Recombinant Protein
  2. Candidate genes on murine chromosome 8 are associated with susceptibility to Staphylococcus aureus infection in mice and are involved with Staphylococcus aureus septicemia in humans
    Authors: Q Yan, SH Ahn, FM Medie, BK Sharma-Kui, LP Park, WK Scott, H Deshmukh, EL Tsalik, DD Cyr, CW Woods, CA Yu, C Adams, R Qi, B Hansen, VG Fowler
    PLoS ONE, 2017;12(6):e0179033.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
    Authors: Tseng C, Kolonin M
    Stem Cells, 2015;34(1):174-90.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF
By Anonymous on 04/30/2019
Application: Binding assay/Protein-protein interaction