Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

R&D Systems | Catalog # 7728-A5

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha 5 beta 1 Protein (7728-A5)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P11688 (Integrin alpha 5) & P09055 (Integrin beta 1)

Structure / Form

Noncovalently-linked heterodimer 

Applications

Bioactivity
View all Integrin alpha 5 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 5 beta 1 protein
Mouse Integrin alpha 5
Phe45-Asn999 (Gly377Ala)
Accession # P11688		 GS Linker Acidic Tail HHHHHH
Mouse Integrin beta 1
(Gln21-Asp728)
Accession # P09055		 HP GS Linker Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe45 (Integrin alpha 5) & Gln21 predicted, No results obtained: sequencing might be blocked (Integrin beta 1)

Predicted Molecular Mass

113 kDa (Integrin alpha 5) & 86.5 kDa (Integrin beta 1)

SDS-PAGE

115-165 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Fibronectin (Catalog # 1030-FN) is coated at 2 μg/mL, Recombinant Mouse Integrin alpha 5 beta 1 binds with an apparent KD <0.5 nM. 

Reviewed Applications

Read 1 review rated 4 using 7728-A5 in the following applications:

Formulation, Preparation, and Storage

7728-A5
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 5 beta 1

Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin  alpha 5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha 5 shares 91% and 97% aa sequence identity with human and rat alpha 5, respectively. Mature mouse Integrin beta 1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta 1 shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 5/ beta 1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14‑16).

References

  1. Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
  2. Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.
  3. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
  4. Takagi, J. et al. (2003) EMBO J. 22:4607.
  5. Wei, Y. et al. (2005) J. Cell Biol. 168:501.
  6. Parsons-Wingerter, P. et al. (2005) Am. J. Pathol. 167:193.
  7. Kim, S. et al. (2000) Am. J. Pathol. 156:1345.
  8. Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
  9. Cascone, I. et al. (2005) J. Cell Biol. 170:993.
  10. Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
  11. Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
  12. Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.
  13. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  14. Carstanjen, D. et al. (2005) Transfusion 45:1192.
  15. Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
  16. Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.

Alternate Names

VLA-5

Entrez Gene IDs

3678 (Human)

Gene Symbol

ITGA5

UniProt

P11688 (Integrin alpha 5) & P09055 (Integrin beta 1)

Additional Integrin alpha 5 beta 1 Products

Product Documents for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

For research use only

Citations for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

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FAQs for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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