Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF Summary
|Mouse Integrin alpha 5
Accession # P11688
|GS Linker||Acidic Tail||HHHHHH|
|Mouse Integrin beta 1
Accession # P09055
|HP||GS Linker||Basic Tail|
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha 5 beta 1
Integrin alpha 5 beta 1, also known as VLA‑5, is a widely expressed non‑covalent heterodimer of a 160 kDa alpha 5 and a 130 kDa beta 1 Integrin subunit. alpha 5/ beta 1 functions in cell adhesion, migration, activation, and matrix fibrillogenesis (1, 2). Mature mouse Integrin alpha 5/CD49e consists of a 955 aa extracellular domain (ECD) with 7 tandem FG‑GAP repeats, a 26 aa transmembrane segment, and a 28 aa cytoplasmic domain (3). Within the ECD, mouse alpha 5 shares 91% and 97% aa sequence identity with human and rat alpha 5, respectively. Mature mouse Integrin beta 1/CD29 consists of a 708 aa ECD with one vWF‑A domain and a cysteine‑rich region, followed by a 23 aa transmembrane segment and a 47 aa cytoplasmic domain (3). Within the ECD, mouse beta 1 shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 5/ beta 1 binds Fibronectin in both RGD‑dependent and ‑independent manners (4, 5). It is up‑regulated on tumor vasculature and promotes angiogenesis (6, 7). This is accomplished in part by a constitutive association in cis of alpha 5/ beta 1 with VEGF R3 and Tie‑2, a requirement for optimal activation of those receptors (8, 9). alpha 5/ beta 1 interacts with a variety of other proteins, including ErbB2, uPAR, Galectin‑1, CTGF, and thrombin‑cleaved Osteopontin (5, 10‑13). alpha 5/ beta 1 also functions on some hematopoietic and neuronal stem cells (14‑16).
- Mao, Y. and Schwarzbauer, J.E. (2005) Matrix Biol. 24:389.
- Gu, J. and Taniguchi, N. (2004) Glycoconj. J. 21:9.
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- Zhang, X. et al. (2004) J. Cell. Physiol. 202:205.
- Cascone, I. et al. (2005) J. Cell Biol. 170:993.
- Kuwada, S.K. et al. (2005) J. Biol. Chem. 280:19027.
- Fischer, C. et al. (2005) J. Biol. Chem. 280:37266.
- Gao, R. and Brigstock, D.R. (2005) Gastroenterology 129:1019.
- Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
- Carstanjen, D. et al. (2005) Transfusion 45:1192.
- Gibson, R.M. et al. (2005) Mol. Cell. Neurosci. 28:588.
- Tanaka, R. et al. (2009) J. Biol. Chem. 284:19817.
Citations for Recombinant Mouse Integrin alpha 5 beta 1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
PLoS ONE, 2022;17(9):e0274667.
Sample Types: Cell Lysates
Applications: ELISA Capture
Galectin-1 Restricts Vascular Smooth Muscle Cell Motility Via Modulating Adhesion Force and Focal Adhesion Dynamics
Authors: MS Tsai, MT Chiang, DL Tsai, CW Yang, HS Hou, YR Li, PC Chang, HH Lin, HY Chen, IS Hwang, PK Wei, CP Hsu, KI Lin, FT Liu, LY Chau
Sci Rep, 2018;8(1):11497.
Sample Types: Recombinant Protein
Candidate genes on murine chromosome 8 are associated with susceptibility to Staphylococcus aureus infection in mice and are involved with Staphylococcus aureus septicemia in humans
Authors: Q Yan, SH Ahn, FM Medie, BK Sharma-Kui, LP Park, WK Scott, H Deshmukh, EL Tsalik, DD Cyr, CW Woods, CA Yu, C Adams, R Qi, B Hansen, VG Fowler
PLoS ONE, 2017;12(6):e0179033.
Sample Types: Whole Cells
Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
Authors: Tseng C, Kolonin M
Stem Cells, 2015;34(1):174-90.
Sample Types: Recombinant Protein
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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