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Bovine Fibronectin Protein, CF

  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically
    1‑5 µg/cm2.  Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
    Optimal concentration depends on cell type as well as the application or research objectives.
  • Source
    Bovine plasma-derived
1030-FN
 
Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 6 months, 2 to 8 °C under sterile conditions after opening.
Avoid vortexing and excessive agitation.
Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

  • References:
    1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
    2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
    3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
    4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
    5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
    6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.
  • Entrez Gene IDs:
    2335 (Human); 280794 (Bovine);
  • Alternate Names:
    FN1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 5 of 5
Filter your results:

Species
Applications
Sample Type
  1. The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.
    Authors: Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX
    PLoS ONE, 2012;7(5):e35030.
    Species: Hamster
    Application: Cell Attachment
  2. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus monkey)
    Application: Bioassay
  3. Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
    Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
    Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104.
    Species: Human
    Application: Bioassay
  4. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Application: Bioassay
  5. IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
    Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
    Eur. J. Immunol., 2008;38(10):2865-73.
    Species: Mouse
    Application: Bioassay

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