Bovine Fibronectin Protein, CF

Catalog # Availability Size / Price Qty
1030-FN-01M
1030-FN-05M
Product Details
Citations (9)
FAQs
Reviews

Bovine Fibronectin Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm2.  Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Bovine plasma-derived Fibronectin protein

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1030-FN

Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 6 months, 2 to 8 °C under sterile conditions after opening.
Avoid vortexing and excessive agitation.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

References
  1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
  2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
  3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
  4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
  5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
  6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.
Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Citations for Bovine Fibronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin
    Authors: N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen
    Acta Neuropathol Commun, 2018;6(1):139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
    Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
    J Neuroinflammation, 2018;15(1):226.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
    Authors: J Yang, C Wu, I Stefanescu, A Horowitz
    J Vis Exp, 2017;0(122):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  4. FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
    Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
    Stem Cells, 2014;32(3):770-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.
    Authors: Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX
    PLoS ONE, 2012;7(5):e35030.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  6. IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
    Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
    Eur. J. Immunol., 2008;38(10):2865-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
    Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
    Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
    Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
    Brain Res. Dev. Brain Res., 2004;152(2):159-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    • Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

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