Recombinant Mouse Integrin alpha X beta 2 Protein, CF

R&D Systems | Catalog # 7987-AX

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha X beta 2 Protein (7987-AX)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q9QXH4 (Integrin alpha X) & P11835 (Integrin beta 2)

Structure / Form

Noncovalently-linked heterodimer

Applications

Bioactivity
View all Integrin alpha X beta 2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha X beta 2 protein
Mouse Integrin alpha X
(Phe20-Pro1116)
Accession # Q9QXH4		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta 2
(Gln24-Asn702)
Accession # P11835		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe20 (Integrin alpha X) & Gln24 predicted, No results obtained: sequencing might be blocked (Integrin beta 2)

Predicted Molecular Mass

131 kDa (Integrin alpha X) & 83 kDa (Integrin beta 2)

SDS-PAGE

135-150 kDa & 95-105 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes.
The ED50 for this effect is 0.750-7.50 μg/mL.

Formulation, Preparation, and Storage

7987-AX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha X beta 2

Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is commonly used as a marker for dendritic cells (DC) and classically activated macrophages (M1), but is also expressed on hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1‑7). Inflammation enhances surface expression of alpha X beta 2 in states such as obesity (adipose DC), asthma (lung DC) and hypertriglyceridemia (circulating monocytes), increasing the adhesive capacity of the cells and contributing to pathology (8‑10). The alpha X vWFA or I‑domain, which contains the adhesion sites, forms the N‑terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain (1, 11). Like other integrins, alpha X beta 2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alpha X beta 2 is fully active and extended. In the inactive state, the heterodimer flexes in the center at the alpha X thigh and calf domains and beta 2 I‑EGF domains, impeding access to adhesion sites (1). The 1097 aa mouse alpha X/CD11c ECD shares 87% aa sequence identity with rat, and 71‑73% with human, canine and equine alpha X, while the 679 aa mouse beta 2/CD18 ECD shares 91% aa sequence identity with rat, and 80-82% with human, bovine, canine, and porcine beta 2 ECD. Active alpha X beta 2 shares some adhesion partners with alpha M beta 2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4-14). However, alpha M beta 2 activity is often constitutive, while alpha X beta 2 activity requires cell activation (4-7). alpha X beta 2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (14-18). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 12, 14, 15). Mutations of beta 2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (19).

References

  1. Corbi, A.L. et al. (1987) EMBO J. 6:4023.
  2. Kishimoto, T.K. et al. (1987) Cell 48:681.
  3. Hynes, R.O. (2002) Cell 110:673.
  4. Arnaout, M.A. (1990) Blood 75:1037.
  5. Postigo, A.A. et al. (1991) J. Exp. Med. 174:1313.
  6. Beyer, M. et al. (2005) Respir. Res. 6:70.
  7. Nicolaou, F. et al. (2003) Blood 101:4033.
  8. Stefanovic-Racic, M. et al. (2012) Diabetes 61:2330.
  9. Gower, R.M. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:160.
  10. van Rijt, L.S. et al. (2005) J. Exp. Med. 201:981.
  11. Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
  12. Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
  13. Pendu, R. et al. (2006) Blood 108:3746.
  14. Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
  15. Schack, L. et al. (2009) J. Immunol. 182:6943.
  16. Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
  17. Gang, J. et al. (2007) Mol. Cells 24:240.
  18. Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
  19. Kishimoto, T.K. et al. (1987) Cell 50:193.

Entrez Gene IDs

3687 (Human)

Gene Symbol

ITGAX

UniProt

Q9QXH4 (Integrin alpha X) & P11835 (Integrin beta 2)

Additional Integrin alpha X beta 2 Products

Product Documents for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

For research use only

Citations for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

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FAQs for Recombinant Mouse Integrin alpha X beta 2 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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