Recombinant Mouse ISM1 Protein, CF

R&D Systems | Catalog # 9846-IS

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse ISM1 Protein (9846-IS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001263418

Applications

Bioactivity
View all Isthmin 1/ISM1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Isthmin 1/ISM1 protein
Ser27-Tyr461, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

50 kDa

SDS-PAGE

61-71 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9846-IS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Isthmin 1/ISM1

Isthimin 1 (ISM1) is adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). Mature mouse ISM1 contains two key domains, a central 45 aa TSP type 1 domain followed by a C-terminal 164 aa AMOP domain, the latter of which is responsible for much of the antiangiogenic function (2). ISM1 was originally characterized in Xenopus, and mouse ISM1 shares a 77% similarity with its frog counterpart. Much greater conservation is seen across mammalian species, with mouse ISM1 sharing 92% and 98% aa sequence identity with human and rat ISM1, respectively. ISM shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, Isthmin 1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of Isthmin 1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble Isthmin 1 induced endothelial cell apoptosis (6). Our in-house bioassay has showed that ISM-1 can support cortical neuron neurite outgrowth.

References

  1. Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
  2. Xiang W. et al. (2011). J. Cell. Mol. Med. 15:359.
  3. Lansdon, L. et al. (2018). Genetics. 208:283.
  4. Osório, L. et al. (2014). Cell cycle. 13:1571.
  5. Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
  6. Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.

Long Name

Isthmin 1

Alternate Names

C20orf82, ISM, ISM-1, ISM1, Isthmin, Isthmin-1

Entrez Gene IDs

140862 (Human); 319909 (Mouse); 311760 (Rat)

Gene Symbol

ISM1

UniProt

NP_001263418

Additional Isthmin 1/ISM1 Products

Product Documents for Recombinant Mouse ISM1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse ISM1 Protein, CF

For research use only

Related Research Areas

Integrins and Associated Molecules

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