Recombinant Mouse ISM1 Protein, CF Summary
- R&D Systems NS0-derived Recombinant Mouse ISM1 Protein (9846-IS)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Ser27-Tyr461, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9846-IS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Isthmin 1/ISM1
Isthimin 1 (ISM1) is adhesion antiangiogenic molecule found in skin, muscosal tissues and immuneregulatory cells (1). Mature mouse ISM1 contains two key domains, a central 45 aa TSP type 1 domain followed by a C-terminal 164 aa AMOP domain, the latter of which is responsible for much of the antiangiogenic function (2). ISM1 was originally characterized in Xenopus, and mouse ISM1 shares a 77% similarity with its frog counterpart. Much greater conservation is seen across mammalian species, with mouse ISM1 sharing 92% and 98% aa sequence identity with human and rat ISM1, respectively. ISM shares various roles in developing embryos, from craniofacial patterning (3) to development of the lung tissues as well as tissue homeostasis in adults (4). In the vascular system, Isthmin 1 has been described as an angiogenesis inhibitor. It inhibits angiogenesis in vitro and in vivo, as well as during tumor progression (5). A dual function of Isthmin 1 has also been described where it exerts pro-survival effect on endothelial cells when immobilized, whereas soluble Isthmin 1 induced endothelial cell apoptosis (6). Our in-house bioassay has showed that ISM-1 can support cortical neuron neurite outgrowth.
- Valle-Rios, R. et al. (2014). J. Interf. Cytok. Res. 34:795.
- Xiang W. et al. (2011). J. Cell. Mol. Med. 15:359.
- Lansdon, L. et al. (2018). Genetics. 208:283.
- Osório, L. et al. (2014). Cell cycle. 13:1571.
- Chen, M. et al. (2014) Cell Death and Differetiation. 21:797.
- Zhang, Y. et al. (2011) Cell Death. Dis. 2:e153.
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