Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
988-VJ-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support adhesion of J45.01 human acute lymphoblastic leukemia T lymphocytes. Liang, T.W. et al. (2002) J. Immunol. 168:1618.

When Recombinant Mouse JAM-B Fc Chimera is immobilized on goat anti-human IgG Fc antibody coated wells, J45.01 cells (100 µL/well at 106 cells/mL) adhesion is induced in a dose dependent manner after a 2 hour incubation at 37 °C. The ED50 for this effect is 10-60 ng/mL.

Source
Mouse myeloma cell line, NS0-derived mouse JAM-B/VE-JAM protein
Mouse JAM-B
(Phe29 - Asn236)
Accession #Q9JI59
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Phe29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa (monomer)
SDS-PAGE
64-70 kDa, reducing conditions

Product Datasheets

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988-VJ

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

988-VJ

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: JAM-B/VE-JAM

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (2, 3, 5). Mouse JAM-B/VE-JAM cDNA predicts a 298 amino acid (aa) residue precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (2, 3). Mouse JAM-B shares approximately 79% aa sequence homology with its human homologue. It also shares approximately 35% aa sequence homology with mouse JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the integrin alpha4beta1. However, the JAM‑B/alpha4beta1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. 
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
  4. Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
  5. Liang, T. et al. (2002) J. Immunol. 168:1618.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.
Long Name
Junctional Adhesion Molecule B
Entrez Gene IDs
58494 (Human); 67374 (Mouse)
Alternate Names
C21orf43; CD322 antigen; CD322; JAM2; JAMB; JAM-B; JAM-BJAM-IT/VE-JAM; junctional adhesion molecule 2JAM-2; junctional adhesion molecule B; PRO245; Vascular endothelial junction-associated molecule; VE-JAM; VE-JAMVEJAMCD322

Citations for Recombinant Mouse JAM-B/VE-JAM Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Transcriptional and Post-Translational Regulation of Junctional Adhesion Molecule-B (JAM-B) in Leukocytes under Inflammatory Stimuli
    Authors: PE Day-Walsh, B Keeble, G Pirabagar, SJ Fountain, PA Kroon
    International Journal of Molecular Sciences, 2022-08-03;23(15):.
    Species: Mouse
    Sample Types: Control
    Applications: Western Blot
  2. Junctional Adhesion Molecule 2 Represents a Subset of Hematopoietic Stem Cells with Enhanced Potential for T Lymphopoiesis
    Authors: V Radulovic, M van der Ga, S Koide, V Sigurdsson, S Lang, S Kaneko, K Miharada
    Cell Rep, 2019-06-04;27(10):2826-2836.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
    Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
    Cell Adh Migr, 2016-04-25;10(4):419-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Function of Jam-B/Jam-C interaction in homing and mobilization of human and mouse hematopoietic stem and progenitor cells.
    Authors: Arcangeli M, Bardin F, Frontera V, Bidaut G, Obrados E, Adams R, Chabannon C, Aurrand-Lions M
    Stem Cells, 2014-04-01;32(4):1043-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and LIF.
    Authors: Su RW, Jia B, Ni H
    PLoS ONE, 2012-04-12;7(4):e34325.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
    Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
    Blood, 2011-08-25;118(17):4609-19.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Adhesion Assay, Flow Cytometry
  7. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009-10-01;128(2):196-205.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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