Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

Name: Recombinant Mouse Lymphotoxin alpha1/beta2 Protein
Brand: R&D Systems
SKU: 9968-LY
Price: 777 USD
Availability: InStock

R&D Systems | Catalog # 9968-LY

Submit a review

Citations (2)

Product Documents

100% Guarantee

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Mouse Lymphotoxin alpha1/beta2 Protein (9968-LY)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P09225 (rmLT alpha); P41155 (rmLT beta)

Structure / Form

GS-linked heterotrimer

Applications

Bioactivity

View all Lymphotoxin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Lymphotoxin protein

Mouse LT alpha
(Lys59-Leu202)
Accession # P09225

GGGGS

Mouse LT beta
(Leu153-Gly306) Accession # P41155

GGGGS

Mouse LT beta
(Leu153-Gly306) Accession # P41155

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys59

Predicted Molecular Mass

50 kDa

SDS-PAGE

42-63 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells.
The ED₅₀ for this effect is 0.3-2.1 ng/mL.

Scientific Data Images for Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

Recombinant Mouse Lymphotoxin alpha1/beta2 Protein Bioactivity

Recombinant Mouse Lymphotoxin alpha 1/ beta 2 (Catalog # 9968-LY) induces NIH‑3T3 mouse embryonic fibroblast cell proliferation. The ED₅₀ for this effect is 0.3-2.1 ng/mL.

Recombinant Mouse Lymphotoxin alpha1/beta2 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Lymphotoxin alpha1/beta2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 42-63 kDa.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9968-LY

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Carrier Free: 9968-LY/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Lymphotoxin

Lymphotoxin alpha (LT alpha) and Lymphotoxin beta (LT beta) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). Mouse LT alpha cDNA encodes a 202 amino acid (aa) secreted soluble protein with a 33 aa signal sequence. The mature mouse LT alpha shares 75% aa sequence identity with human LT alpha (3, 4). Mouse LT beta cDNA encodes a 306 aa type II membrane protein with a 27 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 258 aa extracellular domain. It shares 73% aa sequence identity with human LT beta within common regions of their extracellular domains (5). Secreted LT alpha assembles as a soluble homotrimer, LT alpha 3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha 1/beta 2 and LT alpha 2/beta 1 (5). The soluble LT alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha 1/beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT alpha 2/beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (6, 7). The LT alpha 1/beta 2 heterotrimer binds and activates the LT beta R/TNFRSF3 which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 8, 9). LT beta R also serves as a receptor for LIGHT/TNFSF14 (10). LT alpha 1/beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (11-14). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (15).