Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF

R&D Systems | Catalog # 4735-SE

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse Matriptase/ST14 Catalytic Domain (4735-SE)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_035306

Applications

Enzyme Activity
View all Matriptase/ST14 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse Matriptase/ST14 protein
Gly596-Val855, with an N-terminal Met and 6-His tag
The protein was auto-activated and further purified.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met & Val615

Predicted Molecular Mass

3 kDa & 26 kDa

SDS-PAGE

27 kDa (major) and minor auto-activation fragments, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 4735-SE in the following applications:

Formulation, Preparation, and Storage

4735-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Matriptase/ST14

Matriptase, a mouse type II membrane serine protease encoded by the ST14 (suppression of tumorigenicity 14) gene, is also known as epithin, and membrane-type serine protease 1/MT-SP1 (1-2). Its human ortholog MT-SP1/Matriptase (Catalog # http://www.rndsystems.com/product_results.aspx?k=3946-SE">3946-SE), which shares 81% amino acid identity with epithin, has been thought to play an important role in tumor biology and is a potential target for anti-cancer therapy (3). Matriptase has a multidomain structure containing a putative N-terminal transmembrane region, two CUB domains, four LDLRA repeats, and a C-terminal serine protease domain (1). The protease domain of epithin starts with Val615. R&D Systems recombinant mouse Matriptase is an active protease and consists of the catalytic domain (Val615 to Val855) and a short peptide (Gly596 to Arg614).

References

  1. Cho, E. et al. (2001) J. Biol. Chem. 276:44581.
  2. List, K. et al. (2006) Mol. Med. 12:1.
  3. Uhland, K. (2006) Cell Mol. Life Sci. 63:2968.

Alternate Names

Epithin, MTSP1, PRSS14, SNC19, ST14, TADG-15, TMPRSS14

Entrez Gene IDs

6768 (Human); 19143 (Mouse); 114093 (Rat); 102117178 (Cynomolgus Monkey)

Gene Symbol

ST14

UniProt

NP_035306

Additional Matriptase/ST14 Products

Product Documents for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF

For research use only

Citations for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF

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Protocols

View specific protocols for Recombinant Mouse Matriptase/ST14 Catalytic Domain, CF (4735-SE):

Materials
  • Assay Buffer: 50 mM Tris, 50 mM NaCl, 0.01% (v/v) Tween® 20, pH 9.0
  • Recombinant Mouse Matriptase/ST14 Catalytic Domain (rmMatriptase) (Catalog # 4735-SE)
  • Substrate: BOC-Gln-Ala-Arg-AMC (Catalog # ES014)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rmMatriptase to 0.2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 50 µM in Assay Buffer.
  3. Load into a plate 50 µL of 0.2 ng/µL rmMatriptase, and start the reaction by adding 50 µL of 50 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 50 µM Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A9891).

Per Well:

  • rmMatriptase: 0.010 µg
  • Substrate: 25 µM

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