Recombinant Mouse Mindin Protein, CF Summary
Accession # Q8BMS2
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mindin, also known as Spondin-2 and DIL-1, is a member of the Mindin F-Spondin family of evolutionarily conserved, secreted extracellular matrix proteins. It is composed of an N-terminal F-spondin domain, which mediates integrin binding, and a C-terminal thrombospondin type I repeat, which binds to bacterial lipopolysaccharide (1). Mouse Mindin is synthesized as a 330 amino acid (aa) precursor protein with a molecule weight of approximately 36 kDa (2). The mature mouse protein shares 88% and 99% aa sequence identity with the human and rat orthologs, respectively (2). Mindin is expressed in a variety of tissues in mice with the highest expression levels being detected in lung, spleen, heart, and lymph nodes (2).
Mindin is essential for initiating innate and adaptive immune responses. It has been shown to be a pattern recognition receptor for bacterial pathogens and function as an opsonin for macrophage phagocytosis of bacteria (1, 2). Mindin also binds directly to influenza virus particles and is necessary for macrophage-dependent clearance of influenza viruses from the nasal cavity (3). In addition, Mindin serves as a ligand for leukocyte integrins (1). It is critical for T cell priming and recruitment of macrophages and neutrophils to sites of inflammation (4, 5). Mindin also regulates trafficking of eosinophils and granulocytes into the airspace and plays a role in the development of allergic airways disease (6, 7). Outside the immune system, Mindin has been shown to promote adhesion and outgrowth of hippocampal embryonic neurons, and to be an important mediator of brain ischemic injury and cardiac hypertrophy (8-11). Furthermore, it has been suggested that Mindin can serve as a marker for prostate and ovarian cancer, as well as diabetic nephropathy (12-14).
- Li, Y. et al. (2009) EMBO J. 28:286.
- He, Y.W. et al. (2004) Nat. Immunol. 5:88.
- Jia, W. et al. (2008) J. Immunol. 180:6255.
- Li, H. et al. (2006) EMBO J. 25:4097.
- Jia, W. et al. (2005) Blood 106:3854.
- Li, Z. et al. (2009) J. Leukoc. Biol. 85:124.
- Tighe, R.M. et al. (2011) J. Allergy Ther. 2011 (Suppl. 1). pii: 001.
- Feinstein, Y. et al. (1999) Development 126:3637.
- Yan, L. et al. (2011) Cardiovasc. Res. 92:85.
- Bian, Z.Y. et al. (2012) J. Mol. Med. (Berl.) 90:895.
- Wang, L. et al. (2013) Exp. Neurol. 247:506.
- Simon, I. et al. (2007) Gynecol. Oncol. 106:112.
- Murakoshi, M. et al. (2011) Exp. Diabetes Res. 2011:486305.
- Lucarelli, G. et al. (2013) J. Urol. 190:2271.
Citation for Recombinant Mouse Mindin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The pattern-recognition molecule mindin binds integrin Mac-1 to promote macrophage phagocytosis via Syk activation and NF-?B p65 translocation
Authors: YS Liu, LF Wang, XS Cheng, YN Huo, XM Ouyang, LY Liang, Y Lin, JF Wu, JL Ren, B Guleng
J. Cell. Mol. Med., 2019;0(0):.
Sample Types: Whole Cells
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