Recombinant Mouse Mindin Protein, CF

R&D Systems | Catalog # 8500-SP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Mindin Protein (8500-SP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q8BMS2

Applications

Bioactivity
View all Mindin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Mindin protein
Mouse Mindin
(Gln26-Val330)
Accession # Q8BMS2		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln26

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-47 kDa, reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378.
The ED50 for this effect is 0.3-1.2 μg/mL.

Formulation, Preparation, and Storage

8500-SP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mindin

Mindin, also known as Spondin-2 and DIL-1, is a member of the Mindin F-Spondin family of evolutionarily conserved, secreted extracellular matrix proteins. It is composed of an N-terminal F-spondin domain, which mediates integrin binding, and a C-terminal thrombospondin type I repeat, which binds to bacterial lipopolysaccharide (1). Mouse Mindin is synthesized as a 330 amino acid (aa) precursor protein with a molecule weight of approximately 36 kDa (2).  The mature mouse protein shares 88% and 99% aa sequence identity with the human and rat orthologs, respectively (2). Mindin is expressed in a variety of tissues in mice with the highest expression levels being detected in lung, spleen, heart, and lymph nodes (2).

Mindin is essential for initiating innate and adaptive immune responses. It has been shown to be a pattern recognition receptor for bacterial pathogens and function as an opsonin for macrophage phagocytosis of bacteria (1, 2). Mindin also binds directly to influenza virus particles and is necessary for macrophage-dependent clearance of influenza viruses from the nasal cavity (3). In addition, Mindin serves as a ligand for leukocyte integrins (1). It is critical for T cell priming and recruitment of macrophages and neutrophils to sites of inflammation (4, 5). Mindin also regulates trafficking of eosinophils and granulocytes into the airspace and plays a role in the development of allergic airways disease (6, 7). Outside the immune system, Mindin has been shown to promote adhesion and outgrowth of hippocampal embryonic neurons, and to be an important mediator of brain ischemic injury and cardiac hypertrophy (8-11). Furthermore, it has been suggested that Mindin can serve as a marker for prostate and ovarian cancer, as well as diabetic nephropathy (12-14).

References

  1. Li, Y. et al. (2009) EMBO J. 28:286.
  2. He, Y.W. et al. (2004) Nat. Immunol. 5:88.
  3. Jia, W. et al. (2008) J. Immunol. 180:6255.
  4. Li, H. et al. (2006) EMBO J. 25:4097.
  5. Jia, W. et al. (2005) Blood 106:3854.
  6. Li, Z. et al. (2009) J. Leukoc. Biol. 85:124.
  7. Tighe, R.M. et al. (2011) J. Allergy Ther. 2011 (Suppl. 1). pii: 001.
  8. Feinstein, Y. et al. (1999) Development 126:3637.
  9. Yan, L. et al. (2011) Cardiovasc. Res. 92:85.
  10. Bian, Z.Y. et al. (2012) J. Mol. Med. (Berl.) 90:895.
  11. Wang, L. et al. (2013) Exp. Neurol. 247:506.
  12. Simon, I. et al. (2007) Gynecol. Oncol. 106:112.
  13. Murakoshi, M. et al. (2011) Exp. Diabetes Res. 2011:486305.
  14. Lucarelli, G. et al. (2013) J. Urol. 190:2271.

Alternate Names

DIL1, RG-1, SPON2, Spondin 2

Entrez Gene IDs

10417 (Human); 100689 (Mouse)

Gene Symbol

SPON2

UniProt

Q8BMS2 (Mouse)

Additional Mindin Products

Product Documents for Recombinant Mouse Mindin Protein, CF

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Product Specific Notices for Recombinant Mouse Mindin Protein, CF

For research use only

Citations for Recombinant Mouse Mindin Protein, CF

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