Recombinant Mouse Nephronectin Protein, CF

Catalog # Availability Size / Price Qty
4298-NP-050
Recombinant Mouse Nephronectin Protein Bioactivity
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Product Details
Citations (8)
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Recombinant Mouse Nephronectin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cells/well are added to rmNephronectin coated plates (10 µg/mL,
100 µL/well), approximately 50-80% will adhere after 1 hour at 37 °C.
Source
Mouse myeloma cell line, NS0-derived mouse Nephronectin protein
Asp20-Cys561, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp20
Predicted Molecular Mass
60.3 kDa
SDS-PAGE
80-95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4298-NP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Nephronectin Protein Bioactivity View Larger

Recombinant Mouse Nephronectin (Catalog # 4298-NP) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104cells/well are added to Recombinant Mouse Nephronectin coated plates (10 µg/mL, 100 µL/well), approximately 50-80% will adhere after 1 hour at 37 °C.

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Background: Nephronectin

Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70 - 90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 (1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but downregulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha 8 beta 1 (2). Since deletion of integrin alpha 8 beta 1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8 beta 1 ligand during Nephrogenesis (2, 3).

References
  1. Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
  2. Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
  3. Miner, J. H. (2001) J. Cell Biol. 154:257.
Entrez Gene IDs
255743 (Human); 114249 (Mouse)
Alternate Names
EGFL6L; Nctn; Nephronectin; NPNT; POEM

Citations for Recombinant Mouse Nephronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Pharmacologic inhibition of RGD-binding integrins ameliorates fibrosis and improves function following kidney injury
    Authors: J Basta, L Robbins, L Stout, MJ Prinsen, DW Griggs, M Rauchman
    Physiol Rep, 2020;8(7):e14329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Antibodies against nephronectin ameliorate anti-type II collagen-induced arthritis in mice
    Authors: S Kon, M Honda, K Ishikawa, M Maeda, T Segawa
    FEBS Open Bio, 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA (standard)
  3. Nephronectin is Correlated with Poor Prognosis in Breast Cancer and Promotes Metastasis via its Integrin-Binding Motifs
    Authors: TS Steigedal, J Toraskar, RP Redvers, M Valla, SN Magnussen, AM Bofin, S Opdahl, S Lundgren, BL Eckhardt, JM Lamar, J Doherty, RO Hynes, RL Anderson, G Svineng
    Neoplasia, 2018;20(4):387-400.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  4. Role of integrin alpha8 in murine model of lung fibrosis
    Authors: CF Hung, CL Wilson, YH Chow, LM Schnapp
    PLoS ONE, 2018;13(5):e0197937.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains
    Authors: C Arai, K Yoshizaki, K Miyazaki, K Saito, A Yamada, X Han, K Funada, E Fukumoto, N Haruyama, T Iwamoto, I Takahashi, S Fukumoto
    Sci Rep, 2017;7(0):45181.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Physiological and Pathological Roles in Human Adrenal of the Glomeruli-Defining Matrix Protein NPNT (Nephronectin)
    Authors: AE Teo, S Garg, TI Johnson, W Zhao, J Zhou, CE Gomez-Sanc, M Gurnell, MJ Brown
    Hypertension, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The Role of Nephronectin on Proliferation and Differentiation in Human Dental Pulp Stem Cells
    Authors: J Tang, T Saito
    Stem Cells Int, 2017;2017(0):2546261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys.
    Authors: Zhang H, Tasnim F, Ying JY, Zink D
    Biomaterials, 2009;30(15):2899-911.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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