Recombinant Mouse Netrin-1 Protein, CF

(20 citations)   
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of
    3-200 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse Netrin-1
    (Val22-Ala603)
    Accession # AAC52971
    IEGR GGGSGGGSGGGS 10-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Val22
  • Predicted Molecular Mass
    68.2 kDa
  • SDS-PAGE
    83 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1109-N1/CF
 
1109-N1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images

1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.

Background: Netrin-1

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).

  • References:
    1. Puschel, A. (1999) Mech. Dev. 83:65.
    2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
    3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
    4. Madison, R. et al. (2000) Exp. Neurology 161:563.
    5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
    6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
    7. Corset, V. et al. (2000) Nature 407:747.
  • Entrez Gene IDs:
    9423 (Human); 18208 (Mouse); 396389 (Chicken)
  • Alternate Names:
    netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A novel role for the DNA repair gene Rad51 in Netrin-1 signalling
    Authors: KA Glendining, D Markie, RJ Gardner, EA Franz, SP Robertson, CL Jasoni
    Sci Rep, 2017;7(0):39823.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. An Alzheimer's disease-linked rare mutation potentiates netrin receptor uncoordinated-5C-induced signaling that merges with amyloid ? precursor protein signaling
    Authors: Y Hashimoto, Y Toyama, S Kusakari, M Nawa, M Matsuoka
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Regulation of patterned dynamics of local exocytosis in growth cones by netrin-1.
    Authors: Ros O, Cotrufo T, Martinez-Marmol R, Soriano E
    J Neurosci, 2015;35(13):5156-70.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  4. Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation.
    Authors: Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B
    J Bone Miner Res, 2015;30(5):837-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. The Netrin/RGM receptor, Neogenin, controls adult neurogenesis by promoting neuroblast migration and cell cycle exit.
    Authors: O'Leary C, Bradford D, Chen M, White A, Blackmore D, Cooper H
    Stem Cells, 2015;33(2):503-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons.
    Authors: Lu, Xi, Kim-Han, Jeong So, Harmon, Steve, Sakiyama-Elbert, Shelly E, O'Malley, Karen L
    Mol Neurodegener, 2014;9(0):17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Netrin-1 protects against L-Arginine-induced acute pancreatitis in mice.
    Authors: Chen J, Cai Q, Shen P, Yan R, Wang C, Yang D, Fu H, Chen X
    PLoS ONE, 2012;7(9):e46201.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  9. Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
    Authors: Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A
    J. Neurosci., 2012;32(2):411-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. The Parkinsonian mimetic, MPP+, specifically impairs mitochondrial transport in dopamine axons.
    Authors: Kim-Han JS, Antenor-Dorsey JA, O'Malley KL
    J. Neurosci., 2011;31(19):7212-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
    Authors: Kuwako K, Kakumoto K, Imai T
    Neuron, 2010;67(3):407-21.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  12. Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
    Authors: Tadagavadi RK, Wang W
    J. Immunol., 2010;185(0):3750-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  13. Hypoxia-inducible factor-dependent induction of netrin-1 dampens inflammation caused by hypoxia.
    Authors: Rosenberger P, Schwab JM, Mirakaj V, Masekowsky E, Mager A, Morote-Garcia JC, Unertl K, Eltzschig HK
    Nat. Immunol., 2009;10(2):195-202.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  14. Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.
    Authors: Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L
    Cancer Res., 2009;69(5):1717-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin.
    Authors: Schaffar G, Taniguchi J, Brodbeck T, Meyer AH, Schmidt M, Yamashita T, Mueller BK
    J. Neurochem., 2008;107(2):418-31.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Netrin-1 attenuates ischemic stroke-induced apoptosis.
    Authors: Wu TW, Li WW, Li H
    Neuroscience, 2008;156(3):475-82.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  17. Netrin-1 can affect morphogenesis and differentiation of the mouse mammary gland.
    Authors: Strizzi L, Mancino M, Bianco C, Raafat A, Gonzales M, Booth BW, Watanabe K, Nagaoka T, Mack DL, Howard B, Callahan R, Smith GH, Salomon DS
    J. Cell. Physiol., 2008;216(3):824-34.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  18. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
    Authors: Wang W, Reeves WB, Ramesh G
    Am. J. Physiol. Renal Physiol., 2008;294(4):F739-47.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  19. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Bioassay
  20. Netrin-1 induces angiogenesis via a DCC-dependent ERK1/2-eNOS feed-forward mechanism.
    Authors: Nguyen A, Cai H
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(17):6530-5.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 20 Citations
Primary Antibodies
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WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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ICFlow IC050G  
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Histidine-tagged Protein Purification Resin

IP999 1
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