Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF

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Recombinant Mouse Nope/IGDCC4 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse (rm) Nope/IGDCC4 Fc Chimera at 10 µg/mL (100 µL/well) can bind rmNetrin-4 with a linear range of 8-250 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Nope/IGDCC4 protein
Mouse Nope/IGDCC4
(Gly22-His 953)
Accession # Q9EQS9
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
128.2 kDa (monomer)
160-170 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Nope/IGDCC4

Mouse Nope (Neighbor Of Punc E11) was discovered as a gene proximal to the Punc gene on chromosome 9 (1). Punc and Nope are distant members of a subgroup of the immunoglobulin (Ig) superfamily, which include DCC (Deleted in Colorectal Cancer) (2), Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (rat UNC5H1 and H2, mouse UNC5H2 and H3, and human UNC5H3 and H4) (3), Drosophila Frazzled (4), vertebrate Neogenin (5), and mouse Nope and mouse Punc. Members of this subgroup of the Ig superfamily are type I transmembrane proteins with four Ig domains in their extracellular regions.

Mouse Nope consists of a 21 amino acid (aa) signal peptide, a 933 aa extracellular domain (including four Ig domains, five fibronectin-type III (FnIII) repeats), a 24 aa transmembrane segment, and a 274 aa cytoplasmic domain (1). The extracellular domain of mNope shares 45% aa sequence similarity with mouse Punc. However, the cytoplasmic domains of mNope and mouse Punc do not share aa sequence similarity. Compared to other members of the subgroup of the Ig superfamily, mouse Nope extracellular domain shares 25.8% and 25.2% aa similarity with mouse DCC and mouse Neogenin, respectively. Mouse and human Nope share 90.8% aa sequence similarity. Mouse Nope is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between Nope and the guidance receptor of the DCC family suggest that Nope may have similar functions as the DCC family (6 - 8).

  1. Salbaum, J.M. and C. Kappen (2000) Genomics 64:15.
  2. Fearon et al. (1990) Science 247:49.
  3. Keino-Masu et al. (1996) Cell 87:175.
  4. Hong, K. et al. (1999) Cell 97:927.
  5. Leonardo, E.D. et al. (1997) Nature 386:833.
  6. Kolodziej et al. (1996) Cell 87:197.
  7. Meyerhardt (1997) Oncogene 14:1129.
  8. Vielmetter et al. (1997) Genomics 41:414.
Long Name
Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4
Entrez Gene IDs
57722 (Human); 56741 (Mouse)
Alternate Names
DDM36; hDDM36; IGDCC4; immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily, DCC subclass, member 4; KIAA1628; likely ortholog of mouse neighbor of Punc E11; LOC57722; Neighbor of punc e11; Nope; NOPEFLJ42051; Protein DDM36


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