Recombinant Mouse Osteocrin Protein, CF

Catalog # Availability Size / Price Qty
9700-ON-050
Recombinant Mouse Osteocrin Protein Binding Activity
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Recombinant Mouse Osteocrin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
Source
E. coli-derived mouse Osteocrin protein
Val28-Gly130, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Val28
Predicted Molecular Mass
12 kDa
SDS-PAGE
14-18 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9700-ON

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse Osteocrin Protein Binding Activity View Larger

When Recombinant Mouse NPRC/NPR-3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 µg/mL, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6-3.6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Osteocrin

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acid C-terminal active peptide (1). It was found to modulate osteoblast differentation and to regulate glucose metabolism in muscles (2). Mouse Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and human protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osteocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

References
  1. Nishizawa, H. et al. (2004) Journal of Biological Chemistry, 279:19391.
  2. Thomas, G. et al. (2003) Journal of Biological Chemistry, 278:50563.
  3. Jeramic N. et al. (2017) J Cell Physiol 232:61.
  4. Subbatino, E. et al. (2015) Proc. Acad. Natl. Sci. U. S. A. 112:16042.
  5. Kanai, Y. et al. (2017) J of Clin Invest 127: 4136
  6. Ataman, B. et al. (2016) Nature 539:242.
Entrez Gene IDs
344901 (Human); 239790 (Mouse)
Alternate Names
Musclin; Osteocrin; OSTN

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