Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

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Recombinant Mouse NPRC/NPR3 Fc Chimera Protein Binding Activity
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Product Details
Citations (1)

Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Osteocrin (Catalog # 9700-ON) that produces 50% of the optimal binding response is 0.6-3.6 ng/mL.
Mouse myeloma cell line, NS0-derived mouse NPRC/NPR3 protein
Accession # P70180
Accession #
N-terminal Sequence


Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
78 kDa
76-96 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Mouse NPRC/NPR3 Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse NPRC/NPR3 Fc Chimera (Catalog # 10187-NR) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Mouse Osteocrin (Catalog # 9700-ON) binds with an ED50 of 0.6-3.6 ng/mL.

SDS-PAGE Recombinant Mouse NPRC/NPR3 Fc Chimera Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse INPRC/NPR3 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 76-96 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: NPRC/NPR3

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPR-C or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mouse NPR3 is a type I transmembrane glycoprotein that contains a 438 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 37 aa cytoplasmic region. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

  1. Potter, L.R. et al. (2006) Endocr. Rev. 27:47.
  2. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081.
  3. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563.
  4. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.
Long Name
Natriuretic Peptide Receptor C
Entrez Gene IDs
4883 (Human); 18162 (Mouse); 25339 (Rat)
Alternate Names
ANP-C; ANPR-C; ANPRCFLJ14054; Atrial natriuretic peptide clearance receptor; atrial natriuretic peptide receptor 3; Atrial natriuretic peptide receptor type C; C5orf23; chromosome 5 open reading frame 23; GUCY2B; lgj; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C); NPR3; NPRC; NPR-C; stri

Citation for Recombinant Mouse NPRC/NPR3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Skeletal muscle derived Musclin protects the heart during pathological overload
    Authors: M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke
    Nature Communications, 2022;13(1):149.
    Species: Mouse
    Sample Types: Recombinant Proteins
    Applications: Bioassay


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