Recombinant Mouse Reg3G Protein, CF

Catalog # Availability Size / Price Qty
8189-RG-050
Recombinant Mouse Reg3G Protein, CF Binding Activity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse Reg3G Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.1-0.5 μg/mL.
Source
E. coli-derived mouse Reg3G protein
Glu27-Ala174, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met-Glu27
Predicted Molecular Mass
16 kDa
SDS-PAGE
16 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8189-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Binding Activity View Larger

Recombinant Mouse Reg3G (Catalog # 8189-RG) stimulates cell proliferation of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.1-0.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Reg3G

Regenerating islet-derived protein 3-gamma (Reg3G), also known as pancreatitis-associated protein 3 (PAP3), is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3G is secreted as a 148 amino acid (aa) proprotein, and 11 residues are cleaved from the N-terminus to generate the 15 kDa mature protein (3). This short propeptide serves as an inhibitor that limits the activity of the proprotein (3, 4). Mature mouse Reg3G shares 66% and 88% aa sequence identity with human and rat Reg3G, respectively. Reg3G is expressed by Paneth cells of the small intestine and is released into the gut lumen (5). It is up-regulated in gastrointestinal epithelium by bacterial inflammation or by defects in the mucous lining (5-8). Its expression and secretion is also induced in the lung in response to MRSA (drug resistant S. aureus) and in damaged skeletal muscle and peripheral neurons (4, 9). Reg3G binds to bacterial peptidoglycans, exerts bacteriostatic and bactericidal activities, and protects the mucosa against bacterial invasion (4, 5, 10, 11). It also inhibits inflammation by limiting the number of Th1 cells in the lamina propria (11). R&D in-house data indicate that Reg3G can induce the proliferation of Schwann cells, a property it shares with Reg3B (12).

References
  1. Parikh, A. et al. (2012) Biomol. Concepts 3:57.
  2. Narushima, Y. et al. (1997) Gene 185:159.
  3. Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.
  4. Choi, S-M. et al. (2013) J. Exp. Med. 210:551.
  5. Cash, H.L. et al. (2006) Science 313:1126.
  6. Lee, K.S. et al. (2012) PLoS ONE 7:e30786.
  7. Keilbaugh, S.A. et al. (2005) Gut 54:623.
  8. Burger-van Passen, N. et al. (2012) PLoS ONE 7:e38798.
  9. Klasan, G.S. et al. (2014) Muscle Nerve 49:61.
  10. Brandl, K. et al. (2007) J. Exp. Med. 204:1891.
  11. Vaishnava, S. et al. (2011) Science 334:255.
  12. Livesey, F.J. et al. (1997) Nature 390:614.
Long Name
Regenerating Islet-derived III gamma
Entrez Gene IDs
130120 (Human); 19695 (Mouse); 24620 (Rat)
Alternate Names
LPPM429; MGC118999; MGC119001; Pancreatitis-associated protein 1B; Pancreatitis-associated protein IB; PAP IB; PAP1B; PAP-1B; PAP1BMGC118998; PAP3; PAPIB; PAPIII; Reg III-gamma; Reg3G; REG-3-gamma; regenerating gene III; regenerating islet-derived 3 gamma; regenerating islet-derived protein 3-gamma; Regenerating islet-derived protein III-gamma; REG-III; UNQ429

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Reg3G Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Reg3G Protein, CF and earn rewards!

Have you used Recombinant Mouse Reg3G Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review