Recombinant Mouse S100A9 Protein, CF

Catalog # Availability Size / Price Qty
2065-S9-050
Recombinant Mouse S100A9 Protein, CF Bioactivity
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Recombinant Mouse S100A9 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.036-0.18 μg/mL.
Source
E. coli-derived mouse S100A9 protein
Ala2-Lys113
Accession #
N-terminal Sequence
Analysis
Ala2
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
13 kDa
SDS-PAGE
13 kDa, reducing conditions

Product Datasheets

2065-S9

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and TCEP.
Reconstitution Reconstitute at 300 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Mouse S100A9 Protein, CF Bioactivity View Larger

Recombinant Mouse S100A9 induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.036-0.18 μg/mL.

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Background: S100A9

S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium binding proteins (1, 2). It is
up‑regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid (3-6). Mouse S100A9 contains two EF-hand calcium binding motifs and shares 57% and 79% amino acid sequence identity with human and rat S100A9, respectively (7). S100A9 is a noncovalent homodimer that can also noncovalently heterodimerize with S100A8; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers (8-10). Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue which is not conserved in the mouse protein (11). Human S100A9 can also be modified by S-glutathionylation and oxidation (12). The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does (4). S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes (5, 6, 9, 13). S100A9 also promotes cartilage matrix destruction in osteoarthritis (14) as well as RAGE-dependent cardiac fibrosis and remodeling (6). In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide (15).

References
  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
  3. Edgeworth, J. et al. (1991) J. Biol. Chem. 266:7706.
  4. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  5. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  6. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  7. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
  8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
  9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  11. Edgeworth, J. et al. (1989) Nature 342:189.
  12. Lim, S.Y. et al. (2010) J. Biol. Chem. 285:14377.
  13. Simard, J.-C. et al. (2010) J. Leukoc. Biol. 87:905.
  14. Schelbergen, R.F.P. et al. (2012) Arthritis Rheum. 64:1477.
  15. Zhang, C. et al. (2012) PLoS ONE 7:e32953.
Long Name
S100 Calcium Binding Protein A9
Entrez Gene IDs
6280 (Human); 20202 (Mouse)
Alternate Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9

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