Recombinant Mouse Semaphorin 3B Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
5440-S3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5440-S3-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (4)
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Reviews (1)

Recombinant Mouse Semaphorin 3B Fc Chimera Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 100-400 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 3B protein
Mouse Semaphorin 3B
(Pro26 - Asp722: Arg551Ala, Arg553Ala, Arg554Ala, Arg635Ala, Arg636Ala)
Accession # NP_033179
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Pro26
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
104.2 kDa (monomer)
SDS-PAGE
104-115 kDa, reducing conditions

Product Datasheets

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5440-S3 (with carrier)

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5440-S3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5440-S3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20 with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5440-S3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: Semaphorin 3B

Semaphorin 3B (Sema3B; previously Sem A or Sema V) is one of six Class 3 semaphorins that, in the mouse, share 30% - 55% amino acid (aa) identity (1). Class 3 semaphorins are secreted molecules important for guidance of axons and other cells (2, 3). The 500 aa N-terminal sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure (4). The sema domain is followed by a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, and a basic domain (5). Covalent dimerization (aa 709) are required for activity of Sema3B (5, 6). Mature mouse Sema3B shares 93% aa identity with rat Sema3B, and 86% aa identity with human, bovine, porcine and equine Sema3B. A potential isoform with a nine aa substitution for aa 138 - 158 has been sequenced (7). Like other Class 3 semaphorins, Sema3B signaling is transduced by a transmembrane plexin-A dimer, which also has a Sema domain and is coupled to kinase pathways (2, 3). Sema3B binds indirectly to plexins, and requires interaction with neuropilins for activity (2, 3). Repulsive signals through Neuropilin-1 (Npn-1) and attractive signals through Neuropilin-2 (Npn-2) have been identified, affecting adhesion molecule activity in opposite directions (2, 8). In tumors, Sema3B competes with VEGF165 for binding to Npn-1, which in part downregulates tumor angiogenesis, inhibits tumor cell proliferation and promotes apoptosis (9, 12). However, it is also proposed to promote dissemination and metastatic progression by stimulating Npn-1 mediated IL-8 secretion in a subset of tumors that overexpress Sema3B (13). In differentiating osteoblasts, Sema3B expression is induced by vitamin D and promotes osteoclastogenesis (14).

References
  1. Puschel, A. W. et al. (1995) Neuron 14:941.
  2. Halloran, M. C. and M. A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
  3. Neufeld, G. and O. Kessler (2008) Nat. Rev. Cancer 8:632.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Varshavsky, A. et al. (2008) Cancer Res. 68:6922.
  6. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  7. NCBI protein Accession # EDL21210.
  8. Falk, J. et al. (2005) Neuron 48:63.
  9. Castro-Rivera, E. et al. (2004) Proc. Natl. Acad. Sci. USA 101:11432.
  10. Tomizawa, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98:13954.
  11. Castro-Rivera, E. et al. (2008) Cancer Res. 68:8295.
  12. Koyama, N. et al. (2008) Oncogene 27:6581.
  13. Rolny, C. et al. (2008) J. Exp. Med. 205:1155.
  14. Sutton, A. L. M. et al. (2008) Mol. Endocrinol. 22:1370.
Entrez Gene IDs
7869 (Human); 20347 (Mouse); 363142 (Rat)
Alternate Names
(semaphorin) 3B; LUCA-1; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema V; SemA; SEMA3B; SEMA5; SEMA5FLJ34863; SEMAA; Semaphorin 3B; semaphorin A; semaphorin V; semaphorin-3B; semaphorin-V; SEMAV

Citations for Recombinant Mouse Semaphorin 3B Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Semaphorin3B Promotes Proliferation and Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells in a High-Glucose Microenvironment
    Authors: Q Xing, J Feng, X Zhang
    Stem Cells International, 2021-02-26;2021(0):6637176.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits
    Authors: K Burk, E Mire, A Bellon, M Hocine, J Guillot, F Moraes, Y Yoshida, M Simons, S Chauvet, F Mann
    Nat Commun, 2017-02-22;8(0):14508.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis.
    Authors: Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, Pierani A, Falk J, Moret F, Castellani V
    Nat Commun, 2015-02-27;6(0):6366.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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Recombinant Mouse Semaphorin 3B Fc Chimera Protein
By Anonymous on 05/01/2017
Application: CellProlif
Reason for Rating: It inhibits A549 cell growth but not induces cell death or morphological change, as documented in literature.