Recombinant Mouse Semaphorin 3D Fc Chimera Protein

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.5 -9 µg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Mouse Semaphorin 3D
    (Ser38-Glu738)
    Accession # Q8BH34
    IEGRMDP Mouse IgG2a
    (Glu98-Lys330)
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser38
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    107 kDa
  • SDS-PAGE
    97 - 113 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9386-S3
 
9386-S3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantMouse Semaphorin-3D (Catalog # 9386-S3) binds HUVEC human umbilical vein endothelialcells in a dose-dependent manner. TheED50 for this effect is
1.5 -9 µg/mL.
Background: Semaphorin 3D
Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2).  Class 3 semaphorins are secreted molecules, and contain a common domain structure. The mouse Sema3D domains consist of a 488 aa Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4).  Within these domains, mouse Sema3D shares 94% and 98% aa sequence identity with human and rat Sema3D, respectively.  Semaphorin 3D is a secreted guidance molecule involved in angiogenesis and neuronal development (5).  It induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (6, 7). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (8). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (8).  Semaphorin 3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).
  • References:
    1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
    2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
    3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
    4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
    5. Binch, A. et al. (2015) Oncotarget. 6:18338.
    6. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
    7. Wolman, M.A. et al. (2007) J. Neurobiology. 27: 9653-63.
    8. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.
  • Entrez Gene IDs:
    223117 (Human); 108151 (Mouse); 246262 (Rat)
  • Alternate Names:
    (semaphorin) 3D; coll-2; Collapsin 2; MGC39708; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; SEMA3D; Semaphorin 3D; Semaphorin-3D; Sema-Z2

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