Recombinant Mouse Semaphorin 3D Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
9386-S3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
9386-S3-025
Recombinant Mouse Semaphorin 3D Fc Chimera Protein Bioactivity
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Product Details
Citations (1)
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Recombinant Mouse Semaphorin 3D Fc Chimera Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.5 -9 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Semaphorin 3D protein
Mouse Semaphorin 3D
(Ser38-Glu738)
Accession # Q8BH34
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser38
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
107 kDa
SDS-PAGE
97 - 113 kDa, reducing conditions

Product Datasheets

9386-S3 (with carrier)

9386-S3/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9386-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 400 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9386-S3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Semaphorin 3D Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse Semaphorin-3D (Catalog # 9386-S3) binds HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED50 for this effect is 1.5 -9 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3D

Semaphorin 3D (Sema3D) is a member of the semaphorin family that plays a role in neuronal and vascular development (1, 2).  Class 3 semaphorins are secreted molecules, and contain a common domain structure. The mouse Sema3D domains consist of a 488 aa Sema domain, a 53 aa plexin-semaphorin-integrin (PSI) domain, an 89 aa Ig-like C2-type domain, and a 35 aa basic domain which is proteolytically processed (3, 4).  Within these domains, mouse Sema3D shares 94% and 98% aa sequence identity with human and rat Sema3D, respectively.  Semaphorin 3D is a secreted guidance molecule involved in angiogenesis and neuronal development (5).  It induces the collapse and paralysis of neuronal growth cones and promotes fasciculation of axons (6, 7). Sema3D also inhibits endothelial cell motility and tubulogenesis through cytoskeletal reorganization (2). Binding to Neuropilin-I is required for Sema3D signaling (8). ErbB2 was shown to be a potential Sema3D co-receptor, which is phosphorylated in response to Sema3D in HUVEC cells (8).  Semaphorin 3D inhibition of vascular development facilitates inhibition of glioblastoma growth in the cortex of the brain (1).

References
  1. Sabag, A. et al. (2012) PLoS One. 7:e42912.
  2. Aghajanian, H. et al. (2014) J. Biol. Chem. 289:17971.
  3. Takahashi, K. et al. (2009) Biochimica et Biophysica Acta. 1790:395.
  4. Gu, C. and E. Giraudo. (2013) Exp. Cell Res. 319:1306.
  5. Binch, A. et al. (2015) Oncotarget. 6:18338.
  6. Kuhn, T.B. et al. (2000) J. Neurobiology. 44:126.
  7. Wolman, M.A. et al. (2007) J. Neurobiology. 27: 9653-63.
  8. Aghajanian, H. et al. (2015) Nature Commun. 7:12038.
Entrez Gene IDs
223117 (Human); 108151 (Mouse); 246262 (Rat)
Alternate Names
(semaphorin) 3D; coll-2; Collapsin 2; MGC39708; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; SEMA3D; Semaphorin 3D; Semaphorin-3D; Sema-Z2

Citation for Recombinant Mouse Semaphorin 3D Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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