Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

Catalog # Availability Size / Price Qty
3237-S3-025

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Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit proliferation of the A549 human lung carcinoma cells. The ED50 for this effect is 40-120 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 3F protein
Mouse Semaphorin 3F
Ala19-Pro775 (Arg583Ala and Arg586Ala)
Accession # O88632
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
111.6 kDa (monomer)
SDS-PAGE
110-122 kDa, reducing conditions

Product Datasheets

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3237-S3

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3237-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 3F

Semaphorin 3F (Sema 3F; previously Sema IV) is one of six Class 3 (secreted) semaphorins which in the mouse share 40 - 50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema 3F is expressed in the developing nervous system, especially in the dorsal spinal cord (2, 3). In adults, Sema 3F is expressed in the lung and most other tissues (2). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade beta -propeller similar to that found in integrin molecules. Fourteen conserved cysteine residues and one or more N-glycosylation sites are thought to be critical for forming the secondary structure (4). Isoform A is missing aa 153 - 183 within the Sema domain relative to the long form (isoform B) but appears to have similar activity. C-terminal to the Sema domain, Sema 3F has a basic domain, a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and another basic domain at the C-terminus. Dimerization and cleavage at the C-terminus are required for repulsing activity of class 3 semaphorins (5). Mouse Sema 3F shares 96%, 99%, 92%, 97% and 82% aa identity with human, rat, bovine, canine and chick Sema 3F, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema 3F signaling is transduced by type-A plexins, especially Plexin-A3, via interaction with neuropilin-2 (3, 6). Genetic disruption of either Sema 3F or neuropilin-2 alters motor axon trajectory to the ventral forelimb (3). Sema 3F is deleted or downregulated in many metastatic tumors. Restoration of Sema 3F decreases tumorigenicity, vascularization and adhesiveness, most likely through repulsive interactions, VEGF antagonism and downstream integrin regulation (7).

References
  1. Kruger, R.P. et al. (2005) Nature Rev. Mol. Cell Biol. 6:789.
  2. Eckhardt, F. and A. Meyerhans (1998) Neuroreport 9:3975.
  3. Huber, A.B et al. (2005) Neuron 48:949.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Adams, R.H. et al. (1997) EMBO J. 16:6077.
  6. Yaron, A. et al. (2005) Neuron 45:513.
  7. Chedotal, A. et al. (2005) Cell Death Differ. 12:1044.
Entrez Gene IDs
6405 (Human); 20350 (Mouse); 315996 (Rat)
Alternate Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema III/F; sema IV; Sema3F; SEMA4; SEMA-IV; SEMAK; Semaphorin 3F; semaphorin III/F; semaphorin IV

Citations for Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Ankyrin B Promotes Developmental Spine Regulation in the Mouse Prefrontal Cortex
    Authors: Murphy, KE;Duncan, BW;Sperringer, JE;Zhang, EY;Haberman, VA;Wyatt, EV;Maness, PF;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. In vivo topology converts competition for cell-matrix adhesion into directional migration
    Authors: F Bajanca, N Gouignard, C Colle, M Parsons, R Mayor, E Theveneau
    Nat Commun, 2019-04-03;10(1):1518.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Semaphorin 3F Modulates Corneal Lymphangiogenesis and Promotes Corneal Graft Survival
    Authors: T Reuer, AC Schneider, B Cakir, AD Bühler, JM Walz, T Lapp, C Lange, H Agostini, G Schlunck, C Cursiefen, T Reinhard, F Bock, A Stahl
    Invest. Ophthalmol. Vis. Sci., 2018-10-01;59(12):5277-5284.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis
    Authors: Rainer H Straub
    J. Med. Microbiol., 2016-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
    Authors: Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P
    J Neurosci, 2014-08-20;34(34):11274-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Semaphorin 3F and neuropilin-2 control the migration of human T-cell precursors.
    Authors: Mendes-Da-Cruz D, Brignier A, Asnafi V, Baleydier F, Messias C, Lepelletier Y, Bedjaoui N, Renand A, Smaniotto S, Canioni D, Milpied P, Balabanian K, Bousso P, Lepretre S, Bertrand Y, Dombret H, Ifrah N, Dardenne M, Macintyre E, Savino W, Hermine O
    PLoS ONE, 2014-07-28;9(7):e103405.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. p75NTR is an obligate signaling receptor required for cues that cause sympathetic neuron growth cone collapse.
    Authors: Naska S, Lin DC, Miller FD
    Mol. Cell. Neurosci., 2010-06-17;45(2):108-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF

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Recombinant Mouse Semaphorin 3F Fc Chimera (Truncated), CF
By Anonymous on 09/01/2016
Application: In vitro bioactivity in cell culture