Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10263-S4-050
Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF SDS-PAGE
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Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Semaphorin 4B Fc Chimera (Catalog # 10263-S4), immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 4B protein
Mouse Semaphorin 4B
(Leu31-Glu703)
Accession # Q62179.2
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
102 kDa
SDS-PAGE
101-117 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10263-S4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Semaphorin 4B Fc Chimera (Catalog # 10263-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-117 kDa and 200-230 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4B

Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, mouse Sema4B shares 85% and 95% aa identity with the human and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3-4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).

References
  1. Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
  2. Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
  3. Schultze, W. et al. (2001) J. Neurochem. 78:482.
  4. Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
  5. Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
  6. Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
  7. Nagai, H. et al. (2007) Oncogene. 26:4025.
Entrez Gene IDs
10509 (Human); 20352 (Mouse); 293042 (Rat)
Alternate Names
KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; Semaphorin 4B; semaphorin-4B; SemC

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