Recombinant Mouse Semaphorin 4B Fc Chimera Protein, CF Summary
|Mouse Semaphorin 4B|
Accession # Q62179.2
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Mouse Semaphorin 4B Fc Chimera (Catalog # 10263-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 101-117 kDa and 200-230 kDa, respectively.
Background: Semaphorin 4B
Semaphorin 4B (Sema4B) is a 95-100 kDa, class IV member of the Semaphorin family of proteins (1). Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular domain (ECD) that is characterized by one Sema domain, a PSI region, and an Ig-like C2-type domain. Within the ECD, mouse Sema4B shares 85% and 95% aa identity with the human and rat Sema4B, respectively (1, 2). Sema4B is expressed in hippocampal neurons, glial cells, and immune cells (3-4). It colocalizes and interacts with PSD-95 and participates in the formation or functioning of glutamatergic synapses (4). Sema4B-/- mice display reduced proliferation of astrocytes after CNS injury (5). In the immune system, Sema4B is a negative regulator of basophil-mediated immune response and is implicated in the development of lung cancer (6, 7).
- Gurrapu, S. et al. (2016) Cell Adh. Migr. 10:675.
- Alto, L. and J.R. Terman (2017) Methods. Mol. Biol. 1493:1.
- Schultze, W. et al. (2001) J. Neurochem. 78:482.
- Burkhardt, C. et al. (2005) FEBS Lett. 579:3821.
- Ben-Gigi, L. et al. (2015) eNeuro. 2:1.
- Nakagawa, Y. et al. (2011) J. Immunol. 186:2881.
- Nagai, H. et al. (2007) Oncogene. 26:4025.
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