Recombinant Mouse sFRP-2 Protein

Formulations:
Catalog # Availability Size / Price Qty
1169-FR-025
Product Details
Citations (8)
FAQs
Reviews (2)

Recombinant Mouse sFRP-2 Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm) Frizzled‑1 Fc Chimera (Catalog # 1120-FZ) is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
Source
Mouse myeloma cell line, NS0-derived mouse sFRP-2 protein
Leu25-Cys295, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu25
Predicted Molecular Mass
31.6 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1169-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1169-FR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-2

Secreted Frizzled Related Protein-2 (sFRP-2) belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30 - 35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1, 2). Mature mouse sFRP-2, also known as SARP-1, SDF-5, and FRP-2, shares 99% aa sequence identity with human and rat sFRP-2 (3, 4). sFRP-2 is widely expressed during embryogenesis and in the adult in tissues including the eye, heart, lung, colon, intestine, smooth muscle, pancreas, prostate, testis, kidney, brain, teeth and joints, craniofacial mesenchyme, and preadipocytes (3 - 6). Depending on the context, sFRP-2 can exert either positive or negative effects on Wnt signaling (7 - 9). It also inhibits BMP-induced effects [Alfaro 35646, Oshima 3160]. sFRP-2 can be incorporated into the extracellular matrix through interactions with Fibronectin and Integrin alpha 5 beta 1 (11). sFRP-2 plays a variety of roles during tissue morphogenesis including inhibition of the planar cell polarity pathway and myoblast and osteoblast differentiation (8, 10, 12, 13). sFRP-2 is also expressed in multiple myeloma and glioma in which it promotes tumorigenicity (10, 14). At physiological concentrations sFRP-2 enhances BMP-1 mediated proteolysis of Pro-Collagen I, whereas at higher concentrations it inhibits BMP-1 activity (15, 16). This difference is significant considering that sFRP‑2 is up‑regulated in fibrotic areas of the heart following myocardial infarction (15, 16). Elevated levels of sFRP-2 then promote the recovery of cardiac function by reducing collagen deposition, remodeling, and calcification and by promoting the engraftment of mesenchymal stem cells into the heart (8, 15).

References
  1. Bovolenta, P. et al. (2008) J. Cell Sci. 121:737.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. 94:2859.
  4. Melkonyan, H.S. et al. (1997) Proc. Natl. Acad. Sci. 94:13636.
  5. Hu, E. et al. (1998) Biochem. Biophys. Res. Commun. 247:287.
  6. Leimeister, C. et al. (1998) Mech. Dev. 75:29.
  7. Galli, L.M. et al. (2006) Dev. Dyn. 235:681.
  8. Alfaro, M.P. et al. (2010) J. Biol. Chem. 285:35645.
  9. von Marschall, Z. and L.W. Fisher (2010) Biochem. Biophys. Res. Commun. 400:299.
  10. Oshima, T. et al. (2005) Blood 106:3160.
  11. Lee, J.-L. et al. (2004) J. Biol. Chem. 279:14602.
  12. Sugiyama, Y. et al. (2010) Dev. Biol. 338:193.
  13. Descamps, S. et al. (2008) Cell Tissue Res. 332:299.
  14. Roth, W. et al. (2000) Oncogene 19:4210.
  15. He, W. et al. (2010) Proc. Natl. Acad. Sci. 107:21110.
  16. Kobayashi, K. et al. (2009) Nat. Cell Biol. 11:46.
Long Name
Secreted Frizzled Related Protein 2
Entrez Gene IDs
6423 (Human); 20319 (Mouse)
Alternate Names
FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2

Citations for Recombinant Mouse sFRP-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Identification of genes which regulate stroma-dependent in vitro hematopoiesis
    Authors: P Periasamy, V Tran, HC O'Neill
    PLoS ONE, 2018;13(10):e0205583.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons.
    Authors: Godoy J, Arrazola M, Ordenes D, Silva-Alvarez C, Braidy N, Inestrosa N
    J Biol Chem, 2014;289(52):36179-93.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Wnt and BMP signaling cooperate with Hox in the control of Six2 expression in limb tendon precursor.
    Authors: Yamamoto-Shiraishi Y, Kuroiwa A
    , 2013;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: In vivo
  4. Heparan sulfate 6-O-endosulfatases (Sulfs) coordinate the Wnt signaling pathways to regulate myoblast fusion during skeletal muscle regeneration.
    Authors: Tran, Thanh H, Shi, Xiaofeng, Zaia, Joseph, Ai, Xingbin
    J Biol Chem, 2012;287(39):32651-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Wnt3a binds to several sFRPs in the nanomolar range.
    Authors: Wawrzak D, Metioui M, Willems E, Hendrickx M, de Genst E, Leyns L
    Biochem. Biophys. Res. Commun., 2007;357(4):1119-23.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  6. Sustained interactive Wnt and FGF signaling is required to maintain isthmic identity.
    Authors: Canning CA, Lee L, Irving C, Mason I, Jones CM
    Dev. Biol., 2007;305(1):276-86.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Wnt 6 regulates the epithelialisation process of the segmental plate mesoderm leading to somite formation.
    Authors: Schmidt C, Stoeckelhuber M, McKinnell I, Putz R, Christ B, Patel K
    Dev. Biol., 2004;271(1):198-209.
    Species: Chicken
    Sample Types: In Vivo
    Applications: In Vivo
  8. Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
    Authors: Polesskaya A, Seale P, Rudnicki MA
    Cell, 2003;113(7):841-52.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Mouse sFRP-2 Protein

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Recombinant Mouse sFRP-2 Protein
By Anonymous on 04/13/2017
Application: In vitro bioactivity in cell culture

Downregulation of Wnt-3a in B-16 Murine melanoma cells upon treatment with 200 ng/mL of rm sFRP-2 (#1169-FR). PVDF membrane. Anti-Mouse Wnt-3a at 1:1,000 dilution, secondary antibody at 1:1,000 dilution.


Recombinant Mouse sFRP-2 Protein
By Anonymous on 10/03/2016
Application: In vitro bioactivity in cell culture
Reason for Rating: Great reproducibility.