Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1836-SR-050
Product Details
Citations (1)
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Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 104 cells/well are added to Recombinant Mouse SIGNR1/CD209b Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 30-50% will adhere after 1 hour at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse SIGNR1/CD209b protein
MD Human IgG1
(Pro100-Lys330)
IEGR Mouse SIGNR1
(Ser76-Gly325)
Accession # Q8CJ91
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
55.6 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1836-SR

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIGNR1/CD209b

SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta  and gamma  isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC-SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11-13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).

References
  1. Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
  2. Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
  3. McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
  4. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
  5. Parent, S.A. et al. (2002) Gene 293:33.
  6. Kang, Y.-S. et al. (2003) Int. Immunol. 15:177.
  7. Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327.
  8. Galustian, C. et al. (2003) Int. Immunol. 16:853.
  9. Taylor, P.R. et al. (2004) J. Immunol. 172:1157.
  10. Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
  11. Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962.
  12. Lanoue, A. et al. (2004) J. Exp. Med. 200:1383.
  13. Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
  14. Nagaoka, K. et al. (2005) Int. Immunol. 17:827.
  15. Takahara, K. et al. (2003) Int. Immunol. 16:819.
Long Name
SIGN-related 1
Entrez Gene IDs
69165 (Mouse)
Alternate Names
CD209 antigen like protein B; CD209b; DC SIGN related protein 1; DC SIGNR1; OtB7; SIGNR1

Citation for Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity
    Authors: T Uto, T Fukaya, H Takagi, K Arimura, T Nakamura, N Kojima, B Malissen, K Sato
    Nat Commun, 2016;7(0):11273.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay

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