Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Accession # Q8CJ91
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta and gamma isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC-SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11-13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).
- Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
- Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
- McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
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- Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
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- Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
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Citation for Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity
Authors: T Uto, T Fukaya, H Takagi, K Arimura, T Nakamura, N Kojima, B Malissen, K Sato
Nat Commun, 2016;7(0):11273.
Sample Types: Recombinant Protein
Applications: Enzyme Assay
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