Recombinant Mouse SIGNR7/CD209g Protein, CF

R&D Systems | Catalog # 5198-SR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse SIGNR7/CD209g Protein (5198-SR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

CAL15171

Applications

Bioactivity
View all SIGNR7/CD209g Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SIGNR7/CD209g protein
Arg71-Tyr255 with an N-terminal 6-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

6 His-Arg71

Predicted Molecular Mass

21.9 kDa

SDS-PAGE

29-30 kDa reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of LS180 human colorectal adenocarcinoma cells.
When 5 x 104 cells/well are added to rmSIGN-R7 coated plates (5 μg/mL, 100 μL/well), approximately 70%-90% will adhere after 1 hour at 37° C.  Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

5198-SR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIGNR7/CD209g

SIGNR7 (dendritic cell-specific ICAM-3 grabbing nonintegrin related gene 7), also known as CD209g, belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens. SIGNR7 is one of several mouse proteins which are encoded by separate genes and exhibit structural and sequence homology to human DC-SIGN and DC-SIGNR (1 - 3). Mouse SIGNR7 is a type 2 membrane protein that consists of a 45 amino acid (aa) cytoplasmic domain, a 22 aa transmembrane segment, and a 188 aa extracellular domain with a juxtamembrane neck region and a single carbohydrate recognition domain (CRD). Within the CRD, mouse SIGNR7 shares 44% - 49% aa sequence identity with mouse SIGNR1, 2, 3, 4, and 5 and 81% with SIGNR8. It shares 44% - 54% aa sequence identity with the CRD of bovine SIGNR7, human DC-SIGN, human DC-SIGNR, and rat DC-SIGN. A SIGNR7 ortholog has been described in cow but not in human (4). SIGNR7 binds both fucose and mannose residues and shows a distinct binding preference for the 6’-sulfo-sialyl Lewis× carbohydrate (5). Its interaction with mannosylated BSA is disrupted at low pH (5).

References

  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Cambi, A. and Figdor, C.G. (2005) Curr. Opin. Immunol. 17:345.
  3. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
  4. Yamakawa, Y. et al. (2008) J. Leukoc. Biol. 83:1396.
  5. Powlesland, A.S. et al. (2006) J. Biol. Chem. 281:20440.

Long Name

SIGN-related 7

Alternate Names

CD209g

Entrez Gene IDs

70192 (Mouse)

Gene Symbol

CD209G

UniProt

CAL15171 (Mouse)

Additional SIGNR7/CD209g Products

Product Documents for Recombinant Mouse SIGNR7/CD209g Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SIGNR7/CD209g Protein, CF

For research use only

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