Recombinant Mouse SMOC-2 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse SMOC-2 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.

When 5 x 104 cells/well are added to rmSMOC-2 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37 °C. The ED50 for this effect is 0.2‑0.8 μg/mL.

Mouse myeloma cell line, NS0-derived mouse SMOC-2 protein
Leu30-Gly447, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
47.6 kDa
58-61 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SMOC-2

SMOC-2 (secreted, or SPARC-related, modular calcium-binding protein 2), previously called SMAP2 (smooth muscle-associated protein 2), is a 55 kDa glycoprotein that is a member of the SPARC family of matricellular proteins (1, 2). The mouse SMOC-2 cDNA encodes 447 amino acids (aa), including a 21 aa signal sequence, a Kazal-like domain (aa 40 - 84), two thyroglobulin type-1 segments (aa 87 - 153 and 213 - 281) and two EF-hand sequences (aa 347 - 382 and 384 - 419). Of three splice variants, one shows a 13 aa substitution for aa 443 - 447, another shows an 11 aa insertion after Ala170, and a third contains both of these variations. Mature mouse SMOC-2 shares 99%, 95% and 93% aa identity with rat, human and canine SMOC-2, respectively. SMOC-2 is widely expressed in the extracellular matrix and appears to have adhesion-related functions (1 - 5). Recombinant bacterially produced human SMOC-1 and SMOC-2 can bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin, while keratinocyte SMOC-2 binds integrins alpha v beta 1 and alpha v beta 6 (3, 6). SMOC-2 promotes cell cycle progression by signaling through the integrin-linked kinase (ILK) to upregulate cyclin-D1 (4). When expressed in the endothelial extracellular matrix, it potentiates growth factor-induced angiogenesis (5). SMOC-2 expression is upregulated during neointima formation, promoting proliferation and migration of vascular smooth muscle (2). In the skin, it promotes keratinocyte attachment and migration (6). Since Kazal and thyroglobulin domains are often found in protease inhibitors, SMOC-2 is also proposed to inhibit proteases in the lung and artery (2, 7).

  1. Vannahme, C. et al. (2003) Biochem. J. 373:805.
  2. Nishimoto, S. et al. (2002) Biochim. Biophys. Acta 1576:225.
  3. Novinec, M. et al. (2008) Protein Expr. Purif. 62:75.
  4. Liu, P. et al. (2008) Mol. Biol. Cell 19:248.
  5. Rocnik, E.F. et al. (2006) J. Biol. Chem. 281:22855.
  6. Maier, S. et al. (2008) Exp. Cell Res. 314:2477.
  7. Wilk, J.B. et al. (2007) Am. J. Respir. Crit. Care Med. 175:554.
Long Name
Secreted Modular Calcium-binding Protein 2
Entrez Gene IDs
64094 (Human); 64074 (Mouse); 292401 (Rat)
Alternate Names
bA270C4A.1; bA37D8.1; dJ421D16.1; MST117; MSTP117; MSTP140; SMAP2; SMAP-2; SMAP2Secreted modular calcium-binding protein 2; SMOC2; SMOC-2; smooth muscle associated protein 2; Smooth muscle-associated protein 2; SPARC related modular calcium binding 2; SPARC-related modular calcium-binding protein 2; thyroglobulin type-1 repeat containing protein

Citation for Recombinant Mouse SMOC-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality
    Authors: SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza
    Cell Reports, 2021;35(10):109223.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo


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