Recombinant Mouse Sulfamidase/SGSH Protein, CF

R&D Systems | Catalog # 2969-SU

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse Sulfamidase/SGSH Protein (2969-SU)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

NP_061292

Applications

Enzyme Activity
View all Sulfamidase/SGSH Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Sulfamidase/SGSH protein
Arg23-Leu502, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg23

Predicted Molecular Mass

56 kDa

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >0.25 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

2969-SU
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Sulfamidase/SGSH

Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (1). The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (2). The deduced amino acid sequence of mouse SGSH consists of a signal peptide (residues 1-22) and a mature chain (residues 23-502) (1). Recombinant Mouse Sulfamidase/SGSH corresponds to the mature chain and has sulfatase activity as described in the Activity section.

References

  1. Diez-Roux, G. and Ballabio, A. (2005) Annu. Rev. Genomics Hum. Genet. 6:355.
  2. Gliddon, B.L. et al. (2004) Mol. Genet. Metab. 83:239.

Alternate Names

HSS, MPS3A, SFMD, SGSH

Entrez Gene IDs

6448 (Human); 27029 (Mouse)

Gene Symbol

SGSH

UniProt

NP_061292

Additional Sulfamidase/SGSH Products

Product Documents for Recombinant Mouse Sulfamidase/SGSH Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Sulfamidase/SGSH Protein, CF

For research use only

Citations for Recombinant Mouse Sulfamidase/SGSH Protein, CF

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Protocols

View specific protocols for Recombinant Mouse Sulfamidase/SGSH Protein, CF (2969-SU):

Materials
  • Assay Buffer: 50 mM MES, pH 5.5
  • Recombinant Mouse Sulfamidase/SGSH (mSGSH) (Catalog # 2969-SU)
  • Substrate: 4-Nitrocatechol Sulfate (PNCS), 100 mM stock in deionized water
  • Sodium Hydroxide (NaOH), 2 M stock in deionized water
  • Clear 96-well Plate
  • Plate Reader
  1. Dilute rmSGSH to 40 µg/mL in Assay Buffer.
  2. Dilute Substrate to 20 mM in Assay Buffer.
  3. Combine 150 µL of 40 µg/mL rmSGSH and 150 µL of 20 mM Substrate. Include a Substrate Blank containing 150 µL of Assay Buffer and 150 µL of 20 mM Substrate.
  4. Incubate at 37 °C for 72 hours.
  5. Stop reactions and Substrate Blank by adding 300 µL of 0.2 M NaOH.
  6. Load in a plate 200 µL of each reaction and Substrate Blank.
  7. Read at 510 nm (absorbance) in endpoint mode.
  8. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard P-Nitrocatechol (PNC).

Per Well:
  • rmSGSH: 2 µg
  • Substrate: 5 mM

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