Recombinant Mouse TCAM-1 Protein, CF

R&D Systems | Catalog # 3546-TC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse TCAM-1 Protein (3546-TC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

BAB41106

Applications

Bioactivity
View all TCAM-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse TCAM-1 protein
Ala20-Gly483 & Glu22-Gly483

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20 & Glu22

Predicted Molecular Mass

52 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of MLTC‑1 mouse Leydig tumor cells.
When 5 x 104 cells per well are added to TCAM-1 coated plates (5 μg/mL, 100 μL/well), approximately 65%-85% will adhere after 45 minutes at 37 °C.

Formulation, Preparation, and Storage

3546-TC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCAM-1

Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N-linked glycosylation sites (1). Mouse TCAM-1 is 89% identical to rat TCAM-1. Expression of TCAM-1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).

References

  1. Skatani, S. et al. (2000) Genome 43:957.

Long Name

Testicular Cell Adhesion Molecule 1, TCAM-1P

Alternate Names

TCAM1

Entrez Gene IDs

146771 (Human); 75870 (Mouse); 59305 (Rat)

Gene Symbol

TCAM1P

UniProt

BAB41106

Additional TCAM-1 Products

Product Documents for Recombinant Mouse TCAM-1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TCAM-1 Protein, CF

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

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