Recombinant Mouse TCAM-1 Protein, CF

Catalog # Availability Size / Price Qty
3546-TC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse TCAM-1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of MLTC‑1 mouse Leydig tumor cells. When 5 x 104 cells per well are added to TCAM-1 coated plates (5 μg/mL, 100 μL/well), approximately 65%-85% will adhere after 45 minutes at 37 °C.
Source
Mouse myeloma cell line, NS0-derived mouse TCAM-1 protein
Ala20-Gly483 & Glu22-Gly483
Accession #
N-terminal Sequence
Analysis
Ala20 & Glu22
Predicted Molecular Mass
52 kDa
SDS-PAGE
70-80 kDa, reducing conditions

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3546-TC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3546-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TCAM-1

Mouse TCAM-1 (Testicular cell adhesion molecule-1) is a 59 kDa (predicted) type I transmembrane protein that is a member of the intracellular adhesion molecule (ICAM) family belonging to the immunoglobulin (Ig) superfamily (1). It is synthesized from a 548 amino acid (aa) precursor that contains an 18 aa signal sequence, a 465 aa extracellular region, a 25 aa transmembrane region, and a short cytoplasmic tail of 40 aa. The extracellular region contains five Ig domains and six potential N-linked glycosylation sites (1). Mouse TCAM-1 is 89% identical to rat TCAM-1. Expression of TCAM-1 is detected in the embryo on day 17 and in adult testis, and correlates with the presence of pachytene spermatocytes and spermatids in mouse testis development (1).

References
  1. Skatani, S. et al. (2000) Genome 43:957.
Long Name
Testicular Cell Adhesion Molecule 1, TCAM-1P
Entrez Gene IDs
146771 (Human); 75870 (Mouse); 59305 (Rat)
Alternate Names
TCAM1; TCAM-1

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