Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF

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Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein Binding Activity.
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Product Details

Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Integrin alpha V beta 3  (Catalog # 7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie-1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.
Mouse myeloma cell line, NS0-derived mouse Tie-1 protein
Mouse Tie-1
Accession # Q06806.3
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
106 kDa
105-125 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Mouse Integrin alpha V beta 3 (7889-AV) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse Tie‑1 (1-749) Fc Chimera (Catalog # 11220-TI) binds with an ED50 of 2.00-20.0 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Tie-1 (1-749) Fc Chimera Protein (Catalog # 11220-TI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa and 210-250 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Tie-1

Tyrosine -protein kinase with immunoglobulin (Ig) like and epidermal growth factor (EGF) like domains 1 (Tie1), along with the closely related Tie2, is a transmembrane, vascular-specific receptor tyrosine kinase (RTKs) involved in angiogenesis, vascular development, and hematopoiesis (1,2). The Tie molecules are characterized by three Ig-like domains, three EGF-like domains, and three fibronectin type III-like repeats in the extracellular domain (ECD) and a split tyrosine kinase domain in the cytoplasmic region (3,4). The ECD of mouse Tie1 shares 89% amino acid sequence identity with human Tie1. Tie1 is expressed in endothelial and some hematopoietic progenitor cells and helps modulate angiopoietin signaling (5). While Tie1 is unable to directly bind any of the angiopoietins, it can modulate Tie2/angiopoietin signaling through Tie1/Tie2 heterodimer formation (6). Upregulation of Tie1 has been found in some leukemia and tumor related endothelial cells and inhibition of Tie1 has been shown to prevent metastasis and is being studied as a target for anti-angiogenesis treatments (7-9).

  1. Thomas, M. and Augustin, H. (2009) Angiogenesis 12:125.
  2. Fagiani, E. and Christofori, G. (2013) Cancer Letters 328:1.
  3. Seegar, T.C.M. et al. (2010). Mol. Cell. 37:643.
  4. Barton, W.A. et al. (2006). Nat Struc & Molec Biol. 13:524.
  5. Saharinen, P. et al. (2017) Nat Rev Drug Discov. 16:635.
  6. Yuan, H.T. et al. (2007) FASEB J. 21:3171.
  7. Yang, P. et al. (2015). J. Huazhong Univ. Sci. Technol. Med. Sci. 35:615-622.
  8. D'Amico, G. et al. (2014) J Clin Invest. 124:824.
  9. Khan, K.A. and Kerbel, R.S. (2020) EMBO molecular medicine 12:e12355.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Entrez Gene IDs
7075 (Human); 21846 (Mouse)
Alternate Names
EC 2.7.10; EC; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1


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