Recombinant Mouse TINAGL1 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an
    ED50 = 3-18 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Asp41-His466, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp41
  • Predicted Molecular Mass
    49 kDa
  • SDS-PAGE
    49-58 kDa, reducing conditions
9208-TI
 
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
Recombinant Mouse TINAGL1 (Catalog #
9208-TI) binds to Cultrex Mouse Laminin I in an ELISA Binding Assay. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an ED50 = 3-18 ng/mL.
Background: TINAGL1

Tubulointerstitial Nephritis Antigen-like (TINAGL1), also known as Lipocalin-7, AZ-1, and Arg1, is an approximately 50 kDa matricellular protein that contains a Somatomedin-B like domain, a vWF-C domain, and a cysteine peptidase domain that lacks the critical active site Cys residue (1, 2). Mouse TINAGL1 shares 90% and 97% aa sequence identity with human and rat TINAGL1, respectively. It is a component of the extracellular matrix in basement membranes (3-5) where it binds to Collagens I and IV, Laminin, Integrins a5 and b1, and the Anastellin fragment of Fibronectin (3-5). TINAGL1 promotes cell adhesion and angiogenic sprouting from vascular endothelial cells (3, 6). It is expressed in the adrenal cortex and medulla, vascular smooth muscle cells, cardiac and skeletal muscle cells, and in blastocysts that are competent for implantation (2, 3, 5, 7).

  • References:
    1. Kobayashi, M. et al. (2001) J. Steroid Biochem. Mol. Biol. 77:109.
    2. Wex, T. et al. (2001) Biochemistry 40:1350.
    3. Li, D. et al. (2007) FEBS Lett. 274:2506.
    4. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
    5. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
    6. Brown, L.J. et al. (2010) PLoS One 5:e13905.
    7. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
  • Long Name:
    Tubulointerstitial Nephritis Antigen-like 1
  • Entrez Gene IDs:
    64129 (Human); 94242 (Mouse); 94174 (Rat)
  • Alternate Names:
    androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 20  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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