Recombinant Mouse TINAGL1 Protein, CF

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Recombinant Mouse TINAGL1 Protein Bioactivity
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Product Details

Recombinant Mouse TINAGL1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an
ED50 = 1-6 ng/mL.
Human embryonic kidney cell, HEK293-derived mouse TINAGL1 protein
Asp41-His466, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
49 kDa
49-58 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Mouse TINAGL1 Protein Bioactivity View Larger

Recombinant Mouse TINAGL1 (Catalog # 9208-TI) binds to Cultrex Mouse Laminin I in an ELISA Binding Assay. When Cultrex Mouse Laminin I (Catalog # 3400-010-01) is coated at 5 µg/mL, Recombinant Mouse TINAGL1 binds with an ED50 = 1-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: TINAGL1

Tubulointerstitial Nephritis Antigen-like (TINAGL1), also known as Lipocalin-7, AZ-1, and Arg1, is an approximately 50 kDa matricellular protein that contains a Somatomedin-B like domain, a vWF-C domain, and a cysteine peptidase domain that lacks the critical active site Cys residue (1, 2). Mouse TINAGL1 shares 90% and 97% aa sequence identity with human and rat TINAGL1, respectively. It is a component of the extracellular matrix in basement membranes (3-5) where it binds to Collagens I and IV, Laminin, Integrins a5 and b1, and the Anastellin fragment of Fibronectin (3-5). TINAGL1 promotes cell adhesion and angiogenic sprouting from vascular endothelial cells (3, 6). It is expressed in the adrenal cortex and medulla, vascular smooth muscle cells, cardiac and skeletal muscle cells, and in blastocysts that are competent for implantation (2, 3, 5, 7).

  1. Kobayashi, M. et al. (2001) J. Steroid Biochem. Mol. Biol. 77:109.
  2. Wex, T. et al. (2001) Biochemistry 40:1350.
  3. Li, D. et al. (2007) FEBS Lett. 274:2506.
  4. Tajiri, Y. et al. (2010) Biol. Reprod. 82:263.
  5. Igarashi, T. et al. (2009) Biol. Reprod. 81:948.
  6. Brown, L.J. et al. (2010) PLoS One 5:e13905.
  7. Mukai, K. et al. (2003) J. Biol. Chem. 278:17084.
Long Name
Tubulointerstitial Nephritis Antigen-like 1
Entrez Gene IDs
64129 (Human); 94242 (Mouse); 94174 (Rat)
Alternate Names
androgen-regulated gene 1; ARG1; GIS5; Glucocorticoid-inducible protein 5; LCN7; LIECG3; lipocalin 7; OLRG2; OLRG-2; Oxidized LDL-responsive gene 2 protein; oxidized-LDL responsive gene 2; P3ECSL; TIN Ag-related protein; TINAGL; TINAGL1; TINAG-like 1; TINAGRP; TIN-ag-RP; tubulointerstitial nephritis antigen-like 1; tubulointerstitial nephritis antigen-like; Tubulointerstitial nephritis antigen-related protein


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