Recombinant Mouse TLR12 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
8086-TR-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse TLR12 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant T. gondii Profilin is immobilized at 5 µg/mL (100 µL/well), the concentration of Recombinant Mouse TLR12 Fc Chimera that produces 50% of the optimal binding response is approximately 5-25 ng/mL
Source
Chinese Hamster Ovary cell line, CHO-derived mouse TLR12 protein
Mouse TLR12
(Thr22-Lys709)
Accession # NP_991392
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
102 kDa (monomer)
SDS-PAGE
105-125 kDa, reducing conditions

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8086-TR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8086-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TLR12

Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Within the ectodomain, mouse TLR12 shares 80% aa sequence identity with rat TLR12. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondii infection in vivo (4).

References
  1. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  2. Song, D.H. and J.O. Lee (2012) Immunol. Rev. 250:216.
  3. Zhang, D. et al. (2004) Science 303:1522.
  4. Koblansky, A.A. et al. (2013) Immunity 38:119.
  5. Mishra, B.B. et al. (2008) J. Neuroinflam. 5:53.
  6. Hickey, D.K. et al. (2013) Innate Immun. 19:121.
  7. Kim, S.J. et al. (2012) J. Nutr. Biochem. 23:113.
  8. Andrade, W.A. et al. (2013) Cell Host Microbe 13:42.
  9. Lee, B.L. et al. (2013) eLife 2:e00291.
Long Name
Toll-like Receptor 12
Entrez Gene IDs
384059 (Mouse)
Alternate Names
Gm1365; TLR12; toll-like receptor 12

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