Recombinant Mouse TMEM119 Fc Chimera Protein, CF

R&D Systems | Catalog # 10434-TM

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse TMEM119 Fc Chimera Protein (10434-TM)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q8R138.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TMEM119 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse TMEM119 protein
Mouse TMEM119
(Tyr21-Met91)
Accession # Q8R138.1		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr21

Predicted Molecular Mass

35 kDa

SDS-PAGE

37-60 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Mouse TMEM119 Fc Chimera Protein, CF

Recombinant Mouse TMEM119 Fc Chimera Protein SDS-PAGE

Recombinant Mouse TMEM119 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse TMEM119 Fc Chimera (Catalog # 10434-TM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 37-60 kDa and 70-120 kDa, respectively.

Formulation, Preparation, and Storage

10434-TM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 µg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEM119

TMEM119 (Transmembrane Protein 119, also known as Osteoblast Induction Factor or OBIF), is an approximately 38-kDa type 1 transmembrane protein that is predominantly expressed in osteoblasts and is upregulated during osteoblastic differentiation (1, 2). TMEM119 is also expressed in a cell line of microglia, and TMEM119 immunoreactivity is observed in a specific subset of microglia in brains of neurodegenerative diseases, such as Alzheimer's disease (3). Mature mouse TMEM119 consists of a 71 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain. Within the ECD, mouse TMEM119 shares 78% and 87% aa sequence identity with human and rat TMEM119, respectively. TMEM-119 is involved in the osteoblast differentiation and bone development by acting as a ligand and has been reported to contribute to the proliferation, migration, and invasion of osteosarcoma cells, as well as functioning as an oncogene in osteosarcoma (3, 4).

References

  1. Jiang, Z.H. et al. (2017) Exp. Mol. Med. 49:e329.
  2. Mizuhashi, K. et al. (2012) Dev. Growth Differ. 54:474.
  3. Satoh, J. et al. (2016) Neuropathology 36:39.
  4. Kanamoto, T. et al. (2009) BMC Dev. Biol. 9:70.

Long Name

Transmembrane Protein 119

Alternate Names

OBIF

Entrez Gene IDs

338773 (Human); 231633 (Mouse)

Gene Symbol

TMEM119

UniProt

Q8R138.1

Additional TMEM119 Products

Product Documents for Recombinant Mouse TMEM119 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TMEM119 Fc Chimera Protein, CF

For research use only

Related Research Areas

BMP Family
Osteogenesis Markers

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