Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF Summary
Accession # Q3LRV9
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in PBS.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) binds to apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED50for this effect is 0.45‑4.5 μg/mL.
2 μg/lane of Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-72 kDa and 120-150 kDa, respectively.
TREML4 (Triggering Receptor Expressed on Myeloid cells-like 4), also known as TLT-4, is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mature mouse TREML4 consists of a 172 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 42 aa cytoplasmic domain (1, 2). Within the ECD, mouse TREML4 shares 51% and 76% aa sequence identity with human and rat TREM-2, respectively. TREML4 is expressed in the spleen, CD8 alpha + dendritic cells, and macrophages, and it is capable of recognizing necrotic cells by different phagocytes within the spleen (1). TREML4 is characterized by its Ig-like extracellular domain and short cytoplasmic tail that associates with adaptor DAP12 (2). TREML4 has been shown to play a role in coronary calcification and artery disease (3), and it is also a positive regulator of TLR7 signaling in autoimmunity and antiviral responses (4).
- Hemmi, H. et al. (2009) J. Immunol. 182:1278.
- Hemmi, H. et al. (2012) J. Immunol. 188:1147.
- Sen, S.K. et al. (2014) Am. J. Hum. Genet. 95:66.
- Ramirez-Ortiz, Z. G. et al. (2015) Nat. Immunol. 16:495.
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