Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10172-TL-050
Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF Binding Activity
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Recombinant Mouse TREML4/TLT-4 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED50 for this effect is 0.45-4.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TREML4/TLT-4 protein
Mouse TREML4/TLT-4
(Ser29-Leu197)
Accession # Q3LRV9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa
SDS-PAGE
64-72 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10172-TL

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Binding Activity View Larger

Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL) binds to apoptotic EL-4 mouse lymphoblasts in a flow cytometry assay. The ED50 for this effect is 0.45‑4.5 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TREML4/TLT-4 Fc Chimera (Catalog # 10172-TL)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by Coomassie® Bluestaining, showing bands at 64-72 kDa and 120-150 kDa, respectively.

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Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-like 4), also known as TLT-4, is a type I transmembrane member of the TREM family and Ig superfamily (1, 2). Mature mouse TREML4 consists of a 172 amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aa transmembrane segment, and a 42 aa cytoplasmic domain (1, 2). Within the ECD, mouse TREML4 shares 51% and 76% aa sequence identity with human and rat TREM-2, respectively. TREML4 is expressed in the spleen, CD8 alpha + dendritic cells, and macrophages, and it is capable of recognizing necrotic cells by different phagocytes within the spleen (1). TREML4 is characterized by its Ig-like extracellular domain and short cytoplasmic tail that associates with adaptor DAP12 (2). TREML4 has been shown to play a role in coronary calcification and artery disease (3), and it is also a positive regulator of TLR7 signaling in autoimmunity and antiviral responses (4).

References
  1. Hemmi, H. et al. (2009) J. Immunol. 182:1278.
  2. Hemmi, H. et al. (2012) J. Immunol. 188:1147.
  3. Sen, S.K. et al. (2014) Am. J. Hum. Genet. 95:66.
  4. Ramirez-Ortiz, Z. G. et al. (2015) Nat. Immunol. 16:495.
Long Name
Triggering Receptor Expressed on Myeloid Cells-like 4
Entrez Gene IDs
285852 (Human); 224840 (Mouse); 680862 (Rat)
Alternate Names
TLT4; TREML4

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