Recombinant Mouse TSG-6 Protein, CF

Catalog # Availability Size / Price Qty
2326-TS-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Recombinant Mouse TSG-6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmTSG-6 can bind biotinylated Hyaluronan with an estimated KD <1 nM.
Source
Mouse myeloma cell line, NS0-derived mouse TSG-6 protein
Trp18-Leu275, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Trp18
Predicted Molecular Mass
29.8 kDa
SDS-PAGE
39 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2326-TS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TSG-6

TSG-6 (TNF-stimulated gene 6; also named TNFIP6) is a secreted, 35 - 39 kDa group A member of the LINK-Module superfamily of proteins (1 - 4). Mouse TSG-6 is synthesized as a 275 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 258 aa mature region (5, 6). The mature region has an N-terminal link module (aa 36 - 129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (aa 135 - 246). Link modules bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature mouse TSG-6 shares 97%, 94% and 94% aa identity with rat, human and canine TSG-6, respectively. Cells reported to express TGF-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or as a link between the individual subunits of the extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80 - 85 kDa protein subunit from I alpha I (inter-alpha -inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a catalyst for the removal and transient binding of either HC chain. Each chain is subsequently transferred and covalently-linked to the surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10, 11, 12). This disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).

References
  1. Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
  2. Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
  3. Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
  4. Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
  5. Fulop, C. et al. (1997) Gene 202:95.
  6. Fulop, C. et al. (2003) Development 130:2253.
  7. Kohda, D. et al. (1996) Cell 86:767.
  8. Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
  9. Salustri, A. et al. (2003) Development 131:1577.
  10. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  11. Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
  12. Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
Long Name
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs
7130 (Human); 21930 (Mouse)
Alternate Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

Citations for Recombinant Mouse TSG-6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation
    Authors: MGA Broeren, I Di Ceglie, MB Bennink, PLEM van Lent, WB van den Be, MI Koenders, EN Blaney Dav, PM van der Kr, FAJ van de Loo
    PeerJ, 2018;6(0):e4771.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Endotoxin free hyaluronan and hyaluronan fragments do not stimulate TNF-?, interleukin-12 or upregulate co-stimulatory molecules in dendritic cells or macrophages
    Sci Rep, 2016;6(0):36928.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Hyaluronan Rafts on Airway Epithelial Cells.
    Authors: Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V
    J Biol Chem, 2016;291(3):1448-55.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Endogenous mesenchymal stromal cells in bone marrow are required to preserve muscle function in mdx mice.
    Authors: Fujita R, Tamai K, Aikawa E, Nimura K, Ishino S, Kikuchi Y, Kaneda Y
    Stem Cells, 2015;33(3):962-75.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Acute and temporal expression of tumor necrosis factor (TNF)-alpha-stimulated gene 6 product, TSG6, in mesenchymal stem cells creates microenvironments required for their successful transplantation into muscle tissue.
    Authors: Torihashi S, Ho M, Kawakubo Y, Komatsu K, Nagai M, Hirayama Y, Kawabata Y, Takenaka-Ninagawa N, Wanachewin O, Zhuo L, Kimata K
    J Biol Chem, 2015;290(37):22771-81.
  6. Mesenchymal stem cells inhibit lipopolysaccharide-induced inflammatory responses of BV2 microglial cells through TSG-6.
    Authors: Liu Y, Zhang R, Yan K, Chen F, Huang W, Lv B, Sun C, Xu L, Li F, Jiang X
    J Neuroinflammation, 2014;11(0):135.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Anti-inflammatory recombinant TSG-6 stabilizes the progression of focal retinal degeneration in a murine model.
    Authors: Tuo J, Cao X, Shen D, Wang Y, Zhang J, Oh JY, Prockop DJ, Chan CC
    J Neuroinflammation, 2012;9(0):59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6.
    Authors: Wang N, Li Q, Zhang L, Lin H, Hu J, Li D, Shi S, Cui S, Zhou J, Ji J, Wan J, Cai G, Chen X
    PLoS ONE, 2012;7(8):e43768.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Mouse TSG-6 Protein, CF
By Anonymous on 08/02/2019
Application: In vitro bioactivity in cell culture