Recombinant Mouse TWEAK/TNFSF12 Protein

Formulations:
Catalog # Availability Size / Price Qty
1237-TW-025
Product Details
Citations (12)
FAQs
Reviews

Recombinant Mouse TWEAK/TNFSF12 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.03-0.3 µg/mL.
Source
E. coli-derived mouse TWEAK/TNFSF12 protein
Arg105-His249, with an N-terminal Met and a 6-His tag
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1237-TW

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1237-TW/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TWEAK/TNFSF12

TNF-related weak inducer of apoptosis (TWEAK) is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNFSF12. Mouse TWEAK is a 249 amino acid (aa) protein with an N-terminal 21 aa cytoplasmic domain, a 21 aa transmembrane region and a 204 aa C-terminal extracellular domain (1). The primary structures of the extracellular domains of human and mouse TWEAK are 88% identical. A soluble form of TWEAK is generated from the
membrane-associated molecules by proteolytic cleavage suggesting that TWEAK may have long-range effects. TWEAK is expressed widely in many tissues and cells (1). Although TWEAK has been proposed as a ligand that signals through the death domain receptor 3 (DR3) (2), a TNF receptor superfamily member currently designated TNFRSF25, subsequent studies did not demonstrate binding of TWEAK to cell lines that express DR3 (3). In cells that lack DR3, TWEAK has been shown to bind TWEAK receptor (TWEAK R), a novel TNF receptor superfamily member designated TNFRSF12A (4-7). TWEAK R, also known as fibroblast growth
factor-inducible 14 (Fn14), is a growth factor-inducible immediate-early response gene that is expressed in fibroblasts, hepatocellular carcinomas and endothelial cells. TWEAK-TWEAK R interaction has been shown to promote NF-kappa B activation and mediate multiple cell death pathways. On endothelial cells, TWEAK R plays a role in endothelial cell growth and migration. This effect of TWEAK is not due to up-regulation of VEGF (8).

References
  1. Chicheportiche, Y. et al. (1997) J. Biol Chem. 272:32401.
  2. Marsters, S. et al. (1998) Current Biol. 8:525.
  3. Kaptein, A. et al. (2000) FEBS Letters, 485:135.
  4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
  5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
  6. Feng, S.L. et al. (2000) Am. J. Pathol. 156:1253.
  7. Wiley, S.R. et al. (2001) Immunity 15:837.
  8. Lynch, C. et al. (1998) J. Biol. Chem. 274:8455.
Long Name
TNF-like and Weak Inducer of Apoptosis
Entrez Gene IDs
8742 (Human); 21944 (Mouse); 360548 (Rat)
Alternate Names
APO3 ligand; APO3LMGC20669; DR3LGTWEAKAPO3/DR3 ligand; MGC129581; TNF-related weak inducer of apoptosis; TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; TWEAK

Citations for Recombinant Mouse TWEAK/TNFSF12 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus
    Authors: HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi
    Nat Commun, 2018;9(1):179.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TWEAK/Fn14 mediates atrial-derived HL-1 myocytes hypertrophy via JAK2/STAT3 signalling pathway
    Authors: L Hao, M Ren, B Rong, F Xie, MJ Lin, YC Zhao, X Yue, WQ Han, JQ Zhong
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Fn14 deficiency ameliorates psoriasis-like skin disease in a murine model
    Authors: L Peng, Q Li, H Wang, J Wu, C Li, Y Liu, J Liu, L Xia, Y Xia
    Cell Death Dis, 2018;9(8):801.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. TRAF2 COOPERATES WITH FOCAL ADHESION SIGNALING TO REGULATE CANCER CELL SUSCEPTIBILITY TO ANOIKIS
    Authors: S Daniela da, B Xu, M Maschietto, FA Marchi, MI Alkailani, K Bijian, D Xiao, MA Alaoui-Jam
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. TWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice
    Authors: V Fernández-, C Sastre, N Méndez-Bar, J Egido, JL Martín-Ven, C Gómez-Guer, LM Blanco-Col
    Sci Rep, 2017;7(0):46679.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. TWEAK/Fn14 system and crescent formation in IgA nephropathy.
    Authors: Sasaki Y, Shimizu Y, Suzuki Y, Horikoshi S, Tomino Y
    BMC Nephrol, 2015;16(0):27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Nrf2 protects against TWEAK-mediated skeletal muscle wasting.
    Authors: Al-Sawaf O, Fragoulis A, Rosen C, Kan Y, Sonmez T, Pufe T, Wruck C
    Sci Rep, 2014;4(0):3625.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. TNF-related weak inducer of apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine.
    Authors: Dogra C, Changotra H, Wedhas N, Qin X, Wergedal JE, Kumar A
    FASEB J., 2007;21(8):1857-69.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells.
    Authors: Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A
    Arthritis Res. Ther., 2006;8(5):R146.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation.
    Authors: Polavarapu R, Gongora MC, Winkles JA, Yepes M
    J. Neurosci., 2005;25(44):10094-100.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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