Recombinant Mouse Wnt-16b Protein, CF

  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 2-12 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Asn30-Lys364
  • Accession #
  • N-terminal Sequence
    Analysis
    Asn30
  • Predicted Molecular Mass
    38 kDa
  • SDS-PAGE
    43-63 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9148-WN/CF
 
9148-WN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantMouse Wnt-16b (Catalog # 9148-WN/CF) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells.  The ED50 for this effect is
2-12 µg/mL.
Background: Wnt-16b
Wnt-16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine-rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature mouse Wnt‑16 shares 92% and 97% aa sequence identity with human and rat Wnt‑16, respectively. Wnt-16 is expressed by uterine stromal cells adjacent to the luminal epithelium during implantation (4). It is
up-regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt-16 in mouse embryos and human amniotic fluid (6). It is secreted by osteoblasts and inhibits monocyte differentiation into osteoclasts, thereby contributing to cortical bone thickness and bone mineral density [1279, 7]. Wnt-16 is over-expressed in cells undergoing replicative senescence, and it is up-regulated in articular cartilage by injury and osteoarthritis (9, 10). Wnt-16b expression in skin is up-regulated and enhances cell survival in human basal cell carcinomas (11). Its expression is also up-regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (12). Pre-B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A-Pbx1 fusion protein, also causes up-regulation of Wnt-16 that confers resistance to apoptosis (13, 14). Wnt-16 signaling through both canonical and JNK-mediated (non-canonical) pathways is reported (9-11).
  • References:
    1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
    2. Katoh, H. and M. Katoh (2005) Oncol. Rep. 13:771.
    3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
    4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
    5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
    6. Nath, A.K. et al. (2009) PLoS One 4:e4221.
    7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
    8. Moverare-Skrtic, S. et al. (2014) Nat. Med. 20:1279.
    9. Dell'accio, F. et al. (2008) Arthritis Rheum. 58:1410.
    10. Binet, R. et al. (2009) Cancer Res. 69:9183.
    11. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
    12. Sun, Y. et al. (2012) Nat. Med. 18:1359.
    13. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
    14. Mazieres, J. et al. (2005) Oncogene 24:5396.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 16B
  • Entrez Gene IDs:
    51384 (Human); 93735 (Mouse); 500047 (Rat)
  • Alternate Names:
    WNT16; Wnt16b; Wnt-16b
Related Research Areas

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