Recombinant Mouse Wnt-16b Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
9148-WN-025/CF
Recombinant Mouse Wnt-16b Protein, CF Bioactivity
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Recombinant Mouse Wnt-16b Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 2-12 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-16b protein
Asn30-Lys364
Accession #
N-terminal Sequence
Analysis
Asn30
Predicted Molecular Mass
38 kDa
SDS-PAGE
43-63 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9148-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9148-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Wnt-16b Protein, CF Bioactivity View Larger

RecombinantMouse Wnt-16b (Catalog # 9148-WN/CF) induces differentiation of the C3H10T1/2 mouse mesenchymal stem cell line to alkaline phosphatase secreting osteoblast cells.  The ED50 for this effect is
2-12 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-16b

Wnt-16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine-rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mature mouse Wnt‑16 shares 92% and 97% aa sequence identity with human and rat Wnt‑16, respectively. Wnt-16 is expressed by uterine stromal cells adjacent to the luminal epithelium during implantation (4). It is
up-regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt-16 in mouse embryos and human amniotic fluid (6). It is secreted by osteoblasts and inhibits monocyte differentiation into osteoclasts, thereby contributing to cortical bone thickness and bone mineral density [1279, 7]. Wnt-16 is over-expressed in cells undergoing replicative senescence, and it is up-regulated in articular cartilage by injury and osteoarthritis (9, 10). Wnt-16b expression in skin is up-regulated and enhances cell survival in human basal cell carcinomas (11). Its expression is also up-regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (12). Pre-B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A-Pbx1 fusion protein, also causes up-regulation of Wnt-16 that confers resistance to apoptosis (13, 14). Wnt-16 signaling through both canonical and JNK-mediated (non-canonical) pathways is reported (9-11).

References
  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh (2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS One 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Moverare-Skrtic, S. et al. (2014) Nat. Med. 20:1279.
  9. Dell'accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  10. Binet, R. et al. (2009) Cancer Res. 69:9183.
  11. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  12. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  13. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  14. Mazieres, J. et al. (2005) Oncogene 24:5396.
Long Name
Wingless-type MMTV Integration Site Family, Member 16B
Entrez Gene IDs
51384 (Human); 93735 (Mouse); 500047 (Rat)
Alternate Names
WNT16; Wnt16b; Wnt-16b

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