Recombinant Mouse XCL1/Lymphotactin Protein

R&D Systems | Catalog # 486-LT

R&D Systems
Discontinued Product
486-LT has been discontinued. View all XCL1/Lymphotactin products.

Key Product Details

Source

E. coli

Accession Number

AAA56752.1

Applications

Bioactivity
View all XCL1/Lymphotactin Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse XCL1/Lymphotactin protein
Val22-Gly114, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

10 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1.
The ED50 for this effect is 0.05‑0.1 µg/mL.

Formulation, Preparation, and Storage

486-LT
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: XCL1/Lymphotactin

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant mouse lymphotactin has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

References

  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.

Long Name

Chemokine [C Motif] Ligand 1

Alternate Names

ATAC, Lymphotactin, SCM-1 alpha

Entrez Gene IDs

6375 (Human); 16963 (Mouse)

Gene Symbol

XCL1

UniProt

AAA56752.1

Additional XCL1/Lymphotactin Products

Product Documents for Recombinant Mouse XCL1/Lymphotactin Protein

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Product Specific Notices for Recombinant Mouse XCL1/Lymphotactin Protein

For research use only

Related Research Areas

Chemokines and Receptors

Citations for Recombinant Mouse XCL1/Lymphotactin Protein

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