Recombinant Mouse XCL1/Lymphotactin Protein

Carrier Free

Catalog # Availability Size / Price Qty
486-LT-025/CF

With Carrier

Catalog # Availability Size / Price Qty
486-LT-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (8)
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Recombinant Mouse XCL1/Lymphotactin Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human XCR1. The ED50 for this effect is 0.05‑0.1 µg/mL.
Source
E. coli-derived mouse XCL1/Lymphotactin protein
Val22-Gly114, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
10 kDa

Product Datasheets

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486-LT (with carrier)

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486-LT/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

486-LT

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

486-LT/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: XCL1/Lymphotactin

Mouse lymphotactin (Lptn) and its human homologue (also named human SCM-1 and ATAC) belong to the C or gamma subfamily of chemokines. The C chemokines lack two (the 1st and 3rd) of the four invariant cysteine residues normally found in the CC and CXC chemokines and have an extended carboxy terminus. Mouse lymphotactin encodes a 114 amino acid residue precursor protein with a 21 amino acid residue predicted signal peptide. The expression of lymphotactin is restricted to activated mouse pro-T cells and to activated, class I MHC restricted T cells. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse. Recombinant mouse lymphotactin has been shown to have chemotactic activity for lymphocytes, NK cells and mouse splenocytes.

References
  1. Kelner, G. et al. (1994) Science 266:1395.
  2. Hedrick, J.A. et al. (1997) J. Immunol. 158:1533.
Long Name
Chemokine [C Motif] Ligand 1
Entrez Gene IDs
6375 (Human); 16963 (Mouse)
Alternate Names
ATAC; ATACSmall-inducible cytokine C1; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTNSCM-1-alpha; LTNXC chemokine ligand 1; Lymphotactin; lymphotaxin; SCM-1 alpha; SCM-1; SCM1A; SCM-1a; SCYC1SCM1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); XCL1

Citations for Recombinant Mouse XCL1/Lymphotactin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Anticancer effects of chemokine-directed antigen delivery to a cross-presenting dendritic cell subset with immune checkpoint blockade
    Authors: Y Mizumoto, H Hemmi, M Katsuda, M Miyazawa, Y Kitahata, A Miyamoto, M Nakamori, T Ojima, K Matsuda, M Nakamura, K Hayata, Y Fukuda-Oht, M Sugiyama, T Ohta, T Orimo, S Okura, I Sasaki, K Tamada, H Yamaue, T Kaisho
    Br. J. Cancer, 2020-02-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
    Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
    Front Microbiol, 2019-06-25;10(0):1421.
    Species: Mouse
    Sample Types:
    Applications: Binding Assay
  3. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
    Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
    Cell, 2018-02-08;172(5):1022-1037.e14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The Identification and Distribution of Cattle XCR1 and XCL1 among Peripheral Blood Cells: New Insights into the Design of Dendritic Cells Targeted Veterinary Vaccine
    Authors: K Li, G Wei, Y Cao, D Li, P Li, J Zhang, H Bao, Y Chen, Y Fu, P Sun, X Bai, X Ma, Z Lu, Z Liu
    PLoS ONE, 2017-01-27;12(1):e0170575.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
    Authors: Myster F, Palmeira L, Sorel O, Bouillenne F, DePauw E, Schwartz-Cornil I, Vanderplasschen A, Dewals B
    J Virol, 2015-01-14;89(7):3630-47.
    Species: Ovine
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A parenteral DNA vaccine protects against pneumonic plague.
    Authors: Yamanaka H, Hoyt T, Yang X, Bowen R, Golden S, Crist K, Becker T, Maddaloni M, Pascual DW
    Vaccine, 2010-03-01;28(18):3219-30.
    Applications: ELISA (Standard)
  7. Exercise enhances vaccine-induced antigen-specific T cell responses.
    Authors: Rogers CJ, Zaharoff DA, Hance KW, Perkins SN, Hursting SD, Schlom J, Greiner JW
    Vaccine, 2008-08-14;26(42):5407-15.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. XCL1 (lymphotactin) chemokine produced by activated CD8 T cells during the chronic stage of infection with Mycobacterium tuberculosis negatively affects production of IFN-gamma by CD4 T cells and participates in granuloma stability.
    Authors: Ordway D, Higgins DM, Sanchez-Campillo J, Spencer JS, Henao-Tamayo M, Harton M, Orme IM, Gonzalez Juarrero M
    J. Leukoc. Biol., 2007-08-15;82(5):1221-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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