Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10016-EV-050
Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF Binding Activity
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Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit CCL3/MIP-1 alpha -induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.2-1.2 μg/mL
Source
Human embryonic kidney cell, HEK293-derived r. sanguineus Evasin-1 protein
Human IgG1
(Met-Asp-Pro100 - Lys330)
IEGR R. sanguineus Evasin-1
(Glu21-Asn114)
Accession # P0C8E7.1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met-Asp-Pro100
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
37 kDa
SDS-PAGE
40-61 kDa, reducing conditions

Product Datasheets

10016-EV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

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Binding Activity Recombinant R. sanguineus Evasin-1 Fc Chimera Protein, CF Binding Activity View Larger

Recombinant R.sanguineus Evasin‑1 Fc Chimera (Catalog # 10016-EV) inhibitsRecombinant Human CCL‑3/MIP‑1 alpha -induced chemotaxis of BaF3 mouse pro B cellstransfected with human CCR5. The ED50 for this effect is 0.2‑1.2 μg/mL.

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Background: Evasin-1

Evasin-1 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-1 encodes a 114 amino acid (aa) precursor, which include a 20 aa signal peptide and the 94 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown chemokine neutralization activity. A group of proteins contributing to this activity have been identified as chemokine-binding proteins (CHPBs) and named as Evasins (1-3). Evasin-1 shows high affinity binding to a very limited set of three highly homologous CC chemokines: CCL3, CCL4 and CCL18 (1). Since it is a small molecule, Evasin-1 may be therapeutically useful as novel anti-inflammatory agent.

References
  1. Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
  2. Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
  3. Deruaz, M. et al. (2013) FEBS J. 280:4876.
Long Name
Evasin-1
Alternate Names
Evasin-1

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