Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.
Recombinant R. sanguineus Evasin-3 His-tag Protein, CF
R&D Systems | Catalog # 10034-EV
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Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant R. sanguineus Evasin-3 His-tag Protein (10034-EV)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein
Leu21-Arg86. with a C-terminal 6-His tag
Leu21-Arg86. with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu21
Predicted Molecular Mass
7.8 kDa
SDS-PAGE
8-17 kDa, reducing conditions
Activity
Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 0.15-0.9 μg/mL.
The ED50 for this effect is 0.15-0.9 μg/mL.
Scientific Data Images for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF
Recombinant R. sanguineus Evasin-3 His-tag Protein Bioactivity
Recombinant R. sanguineus Evasin-3 (Catalog # 10034-EV) inhibits IL-8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED50 for this effect is 0.15-0.9 μg/mL.Formulation, Preparation, and Storage
10034-EV
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: Evasin-3
References
- Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
- Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
- Deruaz, M. et al. (2013) FEBS J. 280:4876.
Long Name
Evasin-3
UniProt
Additional Evasin-3 Products
Product Documents for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant R. sanguineus Evasin-3 His-tag Protein, CF
For research use only
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