Recombinant R. sanguineus Evasin-3 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10034-EV-050
Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Bioactivity
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Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit CXCL8-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is 0.15-0.9 μg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived r. sanguineus Evasin-3 protein
Leu21-Arg86. with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu21
Predicted Molecular Mass
7.8 kDa
SDS-PAGE
8-17 kDa, reducing conditions

Product Datasheets

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Bioactivity Recombinant R. sanguineus Evasin-3 His-tag Protein, CF Bioactivity View Larger

Recombinant R. sanguineus Evasin‑3 (Catalog # 10034-EV) inhibits IL‑8/CXCL8-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCXR2. The ED50 for this effect is 0.15-0.9 μg/mL.

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Background: Evasin-3

Evasin-3 is a highly selective chemokine-binding protein isolated from tick saliva. The cDNA of tick Evasin-3 encodes an 86 amino acid (aa) precursor, which includes a 20 aa signal peptide and a 66 aa mature protein (1). Ticks are blood sucking parasites that secrete a wide variety of immunomodulatory proteins to evade the host immune response. The saliva isolated from Ticks has shown to contain chemokine neutralization activity. These proteins have been identified as chemokine binding proteins (CHPBs) that were termed as Evasins (1,2.3). Evasin-3 belongs to a new class of chemokine binding proteins in that it shows high affinity binding to a very limited set of chemokines, including CXCL8 and CXCL1. Since it is very small molecule Evasin-3 may be therapeutically useful as novel anti-inflammatory agent.

References
  1. Frauenschuh, A. et al. (2007) J. Biol. Chem. 282:27250.
  2. Deruaz, M. et al. (2008) J. Exp. Med. 205:2019.
  3. Deruaz, M. et al. (2013) FEBS J. 280:4876.
Long Name
Evasin-3
Alternate Names
Evasin-3

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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