Recombinant Rat Fc gamma RIIB/CD32b Protein, CF Summary
His32-Pro212, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Rat Fc gamma RIIB/CD32b (Catalog # 9666-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50 of 0.5-3 μg/mL.
Background: Fc gamma RIIB/CD32b
Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The Fc gamma RI proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII and RIII proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The mature rat Fc gamma RIIB consists of a 181 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 52 aa cytoplasmic domain. Within the ECD, rat Fc gamma RIIB shares 58% and 78 % aa sequence identity with human and mouse Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).
- Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
- Warncke, M. et al. (2012) J. Immunol. 188:4405.
- Takai, T. et al. (1996) Nature 379:346.
- Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
- Moll, T. et al. (2004) J. Immunol. 173:4724.
- Zhang, Y. et al. (2009) J. Immunol. 182:554.
- Ravetch, J. and L. Lanier (2000) Science 290:84.
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