Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF

Name: Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 583-IC
Price: 312 USD
Availability: InStock

R&D Systems | Catalog # 583-IC

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Rat ICAM-1/CD54 Fc Chimera Protein (583-IC)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q00238

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all ICAM-1/CD54 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat ICAM-1/CD54 protein

Rat ICAM-1 (Gln28 - Thr493) Accession # Q00238	DIEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln28 predicted

Predicted Molecular Mass

78 kDa (monomer)

SDS-PAGE

110-125 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.

When 5 x 10⁴ cells/well are added to rrICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.

Also supports adhesion (40-70%) of PMA treated rat Nb-211 cells when coated at 12.5 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

583-IC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in sterile PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

References

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.