Recombinant Rat MuSK Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9847-MK-050
Recombinant Rat MuSK Fc Chimera Protein Binding Activity
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Recombinant Rat MuSK Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. In the presence of Recombinant Rat Agrin (Catalog # 550-AG), immobilized Recombinant Rat MuSK Fc Chimera (2 µg/mL at 100 µL/well) binds Recombinant Human LRP-4 (Catalog # 5948-LR) with an ED50 of 0.06-0.3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat MuSK protein
Rat MuSK
(Glu22-Thr494)
Accession # Q62838
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
79 kDa
SDS-PAGE
89-104 kDa, reducing conditions

Product Datasheets

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9847-MK

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9847-MK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Rat MuSK Fc Chimera Protein Binding Activity View Larger

When Recombinant Rat MuSK Fc Chimera (Catalog # 9847-MK) is coated at 2 µg/mL, Recombinant Human LRP-4 (Catalog # 5948-LR) binds with an ED50 of 0.06-0.3 µg/mL in the presence of Recombinant Rat Agrin (Catalog # 550-AG).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MuSK

MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). Rat MuSK contains a 473 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, rat MuSK shares 92% and 96% aa sequence identity with human MuSK and mouse MuSK, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). MuSK has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), biglycan (6), and ColQ (7). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (7-8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (9-10).

References
  1. Valenzuela, DM. et al.(1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al. (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.
Long Name
Muscle-specific Receptor Tyrosine Kinase
Entrez Gene IDs
4593 (Human); 18198 (Mouse); 81725 (Rat); 102127677 (Cynomolgus Monkey)
Alternate Names
EC 2.7.10; EC 2.7.10.1; MGC126323; MGC126324; muscle, skeletal, receptor tyrosine kinase; MuSK; skeletal receptor tyrosine-protein kinase

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