Recombinant Rat Neurofascin Protein, CF Summary
Ile25-Ala1031, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neurofascin, a type I transmembrane glycoprotein, is a member of the L1 family of cell adhesion molecules (CAMs) (1-4). L1CAM family members are composed of an extracellular domain (ECD) that contains six immunoglobulin (Ig)-like domains and multiple fibronectin type III repeats, followed by transmembrane and cytoplasmic domains (2, 4, 5). Multiple isoforms of Neurofascin, including NF155, NF166, NF180, and NF186, can be generated by alternative splicing that differ in the combination of fibronectin type III repeats, as well as in the presence of a proline-, alanine-, and threonine-rich segment (PAT domain) located just after the fourth fibronectin type III repeat (4). This recombinant rat Neurofascin protein corresponds to isoform NF155 and shares 98% amino acid sequence identity with comparable regions of human and mouse Neurofascin. In rats, NF155 is transiently expressed by oligodendrocytes and Schwann cells during axon myelination (6, 7). NF155 clusters in paranodal regions of oligodendroglia and binds to the Caspr-Contactin complex located on the adjacent axon to form and stabilize paranodal axoglial junctions (8-11). It has been suggested that the ECD of NF155 must be cleaved from oligodendroglia membranes to form and/or stabilize the paranodal structure (12). NF155 has also been shown to promote neuronal adhesion and neurite outgrowth in rats and chickens (12-14). Alterations in NF155 expression have been associated with multiple sclerosis (15).
- Rathjen, F.G. et al. (1987) Cell 51:841.
- Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
- Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
- Kriebel, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:694.
- Liu, H. et al. (2011) J. Biol. Chem. 286:797.
- Collinson, J.M. et al. (1998) Glia 23:11.
- Basak, S. et al. (2007) Dev. Biol. 311:408.
- Charles, P. et al. (2002) Curr. Biol. 12:217.
- Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
- Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
- Sherman, D.L. et al. (2005) Neuron 48:737.
- Maier, O. et al. (2006) Exp. Cell Res. 312:500.
- Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
- Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
- Howell, O.W. et al. (2006) Brain 129:3173.
Citations for Recombinant Rat Neurofascin Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
IgG4 anti-neurofascin155 antibodies in chronic inflammatory demyelinating polyradiculoneuropathy: Clinical significance and diagnostic utility of a conventional assay
Authors: Katsunori Ikewaki
J. Neuroimmunol., 2016;0(0):.
Neurofascin as target of autoantibodies in Guillain-Barre syndrome.
Authors: Pruss H, Schwab JM, Derst C, Gortzen A, Veh RW
Brain, 2011;134(0):e173; author.
Sample Types: Serum
Applications: ELISA (Capture)
Neurofascin as a novel target for autoantibody-mediated axonal injury.
Authors: Mathey EK, Derfuss T, Storch MK, Williams KR, Hales K, Woolley DR, Al-Hayani A, Davies SN, Rasband MN, Olsson T, Moldenhauer A, Velhin S, Hohlfeld R, Meinl E, Linington C
J. Exp. Med., 2007;204(10):2363-72.
Sample Types: Serum
Applications: ELISA (Capture)
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Rat Neurofascin Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Rat Neurofascin Protein, CF and earn rewards!
Have you used Recombinant Rat Neurofascin Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image