Recombinant SARS-CoV-2 GST-Papain-like Protease, CF Summary
For other Coronavirus proteases and recombinant proteins
Glu746 - Lys1060, with an N-terminal GST-tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 8.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 GST-Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
Background: Papain-like Protease
The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 or Wuhan 2019 coronavirus) is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity. In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways. When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages. This protein contains an N-terminal GST tag.
- Clasman J.R., et al. (2020) Antiviral Res. 174: 104661
- Frieman M., et al. (2009) J. Virol. 83: 6689
- Lindner H.A., et al. (2007) Arch. Biochem. Biophys. 466: 8
- Ratia K., et al. (2014) PLoS Pathog. doi: 10.1371/journal.ppat.1004113
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