Recombinant SARS-CoV-2 GST-Papain-like Protease, CF Summary
Glu746 - Lys1060, with an N-terminal GST-tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 8.0, 300 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
0.5 μg and 1 μg of Recombinant SARS-CoV-2 GST-Papain-like Protease (Catalog # E-611) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing a band at 57 kDa.
Background: Papain-like Protease
The Papain-like protease (PLPro) from the human SARS-CoV-2 coronavirus (Severe Acute Respiratory Syndrome coronavirus 2 is a cysteine protease located within the non-structural protein 3 (NS3) section of the viral polypeptide. In other coronaviruses, PLPro activity is required to process the viral polyprotein into functional, mature subunits; specifically, PLPro cleaves a site at the amino-terminal end of the viral replicase region. In addition to its role in viral protein maturation, PLPro possesses a deubiquitinating and deISG15ylating activity. In vivo, this protease antagonizes innate immunity by acting on IFN beta and NF- kappa B signaling pathways. When used in vitro with polyubiquitin substrates, the enzyme demonstrates a strong preference for K48 linkages. This protein contains an N-terminal GST tag.
- Clasman J.R., et al. (2020) Antiviral Res. 174: 104661
- Frieman M., et al. (2009) J. Virol. 83: 6689
- Lindner H.A., et al. (2007) Arch. Biochem. Biophys. 466: 8
- Ratia K., et al. (2014) PLoS Pathog. doi: 10.1371/journal.ppat.1004113
Citation for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Identification of ebselen and its analogues as potent covalent inhibitors of papain-like protease from SARS-CoV-2
Authors: E Weglarz-To, JM Tomczak, M Talma, M Burda-Grab, M Giurg, S Brul
Scientific Reports, 2021;11(1):3640.
Sample Types: Enzyme
Is Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) a suitable substrate for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?
Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611) only cleaves at the C-terminus of ISG15 and/or Ubiquitin conjugates. Recombinant Human ISG15 Fluorescein Protein, CF (Catalog # UL-613) is not a suitable substrate because the fluorescein-labeled lysine residues are distributed throughout the protein, but not at the C-terminus. Suitable substrate candidates for Catalog #E-611 include include Recombinant Human Ubiquitin AMC Protein, CF (Catalog #U-550), Recombinant Human Ubiquitin Rhodamine 110 Protein, CF (Catalog # U-555), and Recombinant Human ISG15 AMC Protein, CF (Catalog # UL-553).
Is there a molar extinction coefficient for Recombinant SARS-CoV-2 GST-Papain-like Protease, CF (Catalog # E-611)?
We have not determined the molar extinction coefficient of E-611 as this is not a regular QC specification. However, a theoretical E280 extinction coefficient can be produced using Expasy software based on the amino acid sequence, which includes the GST tag in the N-terminus.
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