Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

R&D Systems | Catalog # 10710-CV

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant SARS-CoV Nucleocapsid His-tag Protein (10710-CV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

NP_828858.1
View all Nucleocapsid Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov Nucleocapsid protein
Met1-Ala422, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2, determined by protein ID

Predicted Molecular Mass

47 kDa

SDS-PAGE

41-55 kDa, under reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

10710-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Nucleocapsid

SARS-CoV was discovered in association with cases of severe acute respiratory syndrome (SARS) that infected more than 8,000 persons with over 900 fatalities worldwide in 2002-2003 (1). It belongs to a family of viruses known as coronaviruses that also include MERS and SARS-Cov2 that causes the global pandemic coronavirus disease 2019 (Covid-19). Coronavirus is commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). While the S, E and M proteins build up the viral envelop, the N protein is involved in transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (2, 3). The SARS-CoV N protein shares 90% amino acid sequence identity with SARS-CoV-2 N protein. The N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (4, 5), so it has been used to develop serological diagnostic kit for SARS IgM and IgG antibody tests.

References

  1. Rota, P.A. et al. (2003) Science 300:1394.
  2. Chang, C.K. et al. (2006) J.Biomed. Sci. 13:59.
  3. Hurst, K. R. et al. (2009) J. Virol. 83:7221.
  4. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  5. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

NP_828858.1

Additional Nucleocapsid Products

Product Documents for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

For research use only

Related Research Areas

Infectious Diseases

Customer Reviews for Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF and earn rewards!

Have you used Recombinant SARS-CoV Nucleocapsid His-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes