E6 (Early protein 6) is a viral protein produced in cells infected with the Human Papillomavirus. E6 forms a complex with the host cell ubiquitin ligase E6AP (E6-Associated-Protein) generating a ligase activity that polyubiquitinates tumor suppressors p53 and p73 and targets them to the 26S proteasome for degradation. As a result DNA damage and chromosomal instabilities increase, often leading to cell proliferation and cancer. The E6/E6AP complex also targets other substrates for ubiquitination, such as TERT, BAK1, FADD, and pro-CASP8—none of which appear to be substrates for E6AP in the absence of E6.
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Recombinant Viral HPV E6 Protein, CF
R&D Systems | Catalog # AP-120
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Key Product Details
- R&D Systems E. coli-derived Recombinant Viral HPV E6 Protein (AP-120)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived viral E6 protein
Met1-Leu158 with a N-terminal Ser-His
Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118, 147
Met1-Leu158 with a N-terminal Ser-His
Contains Cys to Ser substitutions at the following positions: 23, 58, 87, 104, 118, 147
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Predicted Molecular Mass
19 kDa
Activity
Typical concentration to support E6AP-mediated p53 ubiquitination in vitro is 0.1-2 μM depending on experimental conditions.
Formulation, Preparation, and Storage
AP-120
| Formulation | Supplied as a solution in HEPES, NaCl, DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: E6
References
-
Beaudenon S. & Huibregtse J.M. (2008) BMC Biochem. 9 (Supp1): S4.
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Zanier K., et al. (2007) Prot. Exp. & Purif. 51: 59.
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Zanier K., et al. (2010) J. Mol. Biol. 396: 90.
Long Name
Early Protein 6
Alternate Names
HpV16gp1
Entrez Gene IDs
1489078 (Viral)
Gene Symbol
E6
UniProt
Additional E6 Products
Product Documents for Recombinant Viral HPV E6 Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Viral HPV E6 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Viral HPV E6 Protein, CF
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