Recombinant Viral JEV-NS1 Protein, CF

R&D Systems | Catalog # 9775-JV

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Viral JEV-NS1 Protein (9775-JV)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P32886
View all Japanese Encephalitis virus NS1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral Japanese Encephalitis virus NS1 protein
Thr796-Ala1146, with C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr796

Predicted Molecular Mass

41 kDa

SDS-PAGE

45-52 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9775-JV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Japanese Encephalitis virus NS1

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References

  1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Alternate Names

JE virus, JEV, JEV-NS1, JEVgp1

Entrez Gene IDs

1489713 (Viral)

Gene Symbol

POLY

UniProt

P32886

Additional Japanese Encephalitis virus NS1 Products

Product Documents for Recombinant Viral JEV-NS1 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Viral JEV-NS1 Protein, CF

For research use only

Customer Reviews for Recombinant Viral JEV-NS1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Viral JEV-NS1 Protein, CF and earn rewards!

Have you used Recombinant Viral JEV-NS1 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes